| TAZ-TFG-2018-2338 |
Fabricación y evaluación de nanoestructuras de ADN sensibles al pH para su uso en nanomedicina
Jover Bravo, Inés
Hernández Aínsa, Silvia (dir.)
Serrano Ostáriz, Jósé Luis (ponente)
Universidad de Zaragoza,
CIEN,
2018
Química Orgánica department, Química Orgánica area
Graduado en Biotecnología
Tipo de Trabajo Académico: Trabajo Fin de Grado
Notas: The paramountcy of early diagnosis and good prognosis of cancer has lead much of the on-going research towards the development of novel technology and advanced materials. Among others, DNA as building material present properties such as biocompatibility and reliable design that make it ideal for targeted tumour imaging. Recent studies have been instrumental in strengthening programmable pH responsive nanoswitches for their potential use as biosensors of tumour microenvironment regardless of their liability to suffer from quick clearance and nucleases degradation. The aim of this research is to overcome the aforementioned hindrances fabricating and evaluating new DNA nanostructures incorporating the formerly studied nanoswitches, together with ascertaining whether or not any of these nanostructures could be suitable for their in vivo application. To that end, both the structural and optical characterization in addition to the stability to nucleases assessment were performed. Ultimately, this essay has served to suggest the complex 8SN3 as the best candidate for further in vivo studies found on its resistance to temperature and nucleases as well as its pH transition, which best meets the in vivo sensing requirements for bioimaging.
Notas: The paramountcy of early diagnosis and good prognosis of cancer has lead much of the on-going research towards the development of novel technology and advanced materials. Among others, DNA as building material present properties such as biocompatibility and reliable design that make it ideal for targeted tumour imaging. Recent studies have been instrumental in strengthening programmable pH responsive nanoswitches for their potential use as biosensors of tumour microenvironment regardless of their liability to suffer from quick clearance and nucleases degradation. The aim of this research is to overcome the aforementioned hindrances fabricating and evaluating new DNA nanostructures incorporating the formerly studied nanoswitches, together with ascertaining whether or not any of these nanostructures could be suitable for their in vivo application. To that end, both the structural and optical characterization in addition to the stability to nucleases assessment were performed. Ultimately, this essay has served to suggest the complex 8SN3 as the best candidate for further in vivo studies found on its resistance to temperature and nucleases as well as its pH transition, which best meets the in vivo sensing requirements for bioimaging.
Permalink:
El registro pertenece a las siguientes colecciones:
trabajos-academicos-universidad-zaragoza > centro > facultad-de-ciencias
trabajos-academicos-universidad-zaragoza > trabajos-fin-grado