156575 20251017144604.0 doi 10.2217/nnm-2019-0371 sideral 118681 ART-2020-118681 eng Lacoma, Alicia Novel intracellular antibiotic delivery system against Staphylococcus aureus: Cloxacillin-loaded poly(d, l-lactide-co-glycolide) acid nanoparticles 2020 Access copy available to the general public Unrestricted Aim: First, to compare in vitro minimum inhibitory concentrations (MIC) of free cloxacillin and cloxacillin-containing nanoparticles (NP) against methicillin-susceptible (MSSA) and resistant Staphylococcus aureus (MRSA) and second, to assess NP antimicrobial activity against intracellular S. aureus. Methods: Poly(d, l-lactide-co-glycolide) acid (PLGA)-NP were loaded with cloxacillin and physico-chemically characterized. MICs were determined for reference strains Newman-(MSSA) and USA300-(MRSA). Murine alveolar macrophages were infected, and bacterial intracellular survival was assessed after incubating with free-cloxacillin or PLGA-cloxacillin-NP. Results & conclusion: For both isolates, MICs for antibiotic-loaded-NP were lower than those obtained with free cloxacillin, indicating that the drug encapsulation improves antimicrobial activity. A sustained antibiotic release was demonstrated when using the PLGA-cloxacillin-NP. When considering the lowest concentrations, the use of drug-loaded NP enabled a higher reduction of intracellular bacterial load. info:eu-repo/grantAgreement/EUR/ERC-2013-CoG-614715-NANOHEDONISM info:eu-repo/grantAgreement/ES/ISCIII/FEDER/PI17-01139 info:eu-repo/grantAgreement/ES/MICINN/CTQ2017-84473-R info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 5.307 2020 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 30 / 158 = 0.19 2020 Q1 T1 NANOSCIENCE & NANOTECHNOLOGY 46 / 106 = 0.434 2020 Q2 T2 0.946 2020 Bioengineering 2020 Q1 Biomedical Engineering 2020 Q1 Nanoscience and Nanotechnology 2020 Q1 Materials Science (miscellaneous) 2020 Q1 Medicine (miscellaneous) 2020 Q1 Development 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Usón, Laura Universidad de Zaragoza (orcid)0000-0002-4678-7465 Mendoza, Gracia Universidad de Zaragoza (orcid)0000-0003-2293-363X Sebastián, Víctor Universidad de Zaragoza (orcid)0000-0002-6873-5244 Garcia-Garcia, Esther Muriel-Moreno, Beatriz Domínguez, José Arruebo, Manuel Universidad de Zaragoza (orcid)0000-0003-3165-0156 Prat, Cristina 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 15, 12 (2020), 1189-1203 Nanomedicine Nanomedicine 1743-5889 9881300 http://zaguan.unizar.es/record/156575/files/texto_completo.pdf Versión publicada 1200034 http://zaguan.unizar.es/record/156575/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:156575 articulos driver 2025-10-17-14:14:51 ARTICLE