000156599 001__ 156599
000156599 005__ 20251017144623.0
000156599 0247_ $$2doi$$a10.1021/acsami.5c01920
000156599 0248_ $$2sideral$$a143931
000156599 037__ $$aART-2025-143931
000156599 041__ $$aeng
000156599 100__ $$0(orcid)0000-0002-4261-9480$$aAlleva, María
000156599 245__ $$aFörster resonance energy Transfer (FRET) demonstrates in vitro chitosan-coated nanocapsules suitability for intranasal brain delivery
000156599 260__ $$c2025
000156599 5060_ $$aAccess copy available to the general public$$fUnrestricted
000156599 5203_ $$aIntranasal drug delivery to the brain offers a promising strategy to overcome biological barriers. Chitosan-coated nanoemulsion-based nanocapsules demonstrate significant potential due to their mucoadhesive properties, ability to permeate epithelial cells, and ability to solubilize poorly water-soluble drugs, making them ideal candidates for bypassing the blood-brain barrier and overcoming the nasal mucosa. To ensure effective drug delivery, it is critical to assess the integrity of these nanocapsules during their transit across such barriers. In this study, we employed Förster resonance energy transfer to track the structural integrity of nanocapsules during transport. A simplified in vitro model was established using Calu-3 cells to mimic the mucosal epithelial barrier and Balb-c 3T3 fibroblasts as target cells. Our findings demonstrated that the nanoemulsion core of the nanocapsules successfully crossed the in vitro epithelial barrier and reached target cells while maintaining its structural integrity. These results validate the potential of chitosan-coated nanocapsules as a robust platform for the intranasal delivery of drugs to the brain.
000156599 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E15-20R$$9info:eu-repo/grantAgreement/EC/H2020/842652 /EU/Targeted intracellular delivery of antitubercular agents by functionalized nanocapsules/TBNANO$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 842652 -TBNANO$$9info:eu-repo/grantAgreement/ES/ISCIII/CB16-01/00263$$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-118485RB-I00$$9info:eu-repo/grantAgreement/ES/MICIU/CEX2023-001286-S
000156599 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000156599 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000156599 700__ $$0(orcid)0000-0002-2174-6747$$aBaranyai, Zsuzsa
000156599 700__ $$aEsteban-Pérez, Natalia
000156599 700__ $$aMartínez-Vicente, Pablo$$uUniversidad de Zaragoza
000156599 700__ $$0(orcid)0000-0003-0702-8260$$aMartín-Rapún, Rafael$$uUniversidad de Zaragoza
000156599 700__ $$0(orcid)0000-0002-2861-2469$$aMoros, María
000156599 700__ $$0(orcid)0000-0003-1081-8482$$aMartínez de la Fuente, Jesús
000156599 7102_ $$11003$$2027$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Anatom.Embriol.Humana
000156599 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000156599 773__ $$g17, 18 (2025), 26348-26360$$pACS appl. mater. interfaces$$tACS applied materials & interfaces$$x1944-8244
000156599 8564_ $$s6003777$$uhttps://zaguan.unizar.es/record/156599/files/texto_completo.pdf$$yVersión publicada
000156599 8564_ $$s3142714$$uhttps://zaguan.unizar.es/record/156599/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000156599 909CO $$ooai:zaguan.unizar.es:156599$$particulos$$pdriver
000156599 951__ $$a2025-10-17-14:22:39
000156599 980__ $$aARTICLE