000156603 001__ 156603
000156603 005__ 20251017144626.0
000156603 0247_ $$2doi$$a10.1093/jac/dkaf104
000156603 0248_ $$2sideral$$a143923
000156603 037__ $$aART-2025-143923
000156603 041__ $$aeng
000156603 100__ $$aCampaña-Burguet, Allelen
000156603 245__ $$aChanging epidemiology of MRSA-CC398 in Spanish hospitals located in areas with different pig farming densities: a 2023 multicentre study
000156603 260__ $$c2025
000156603 5203_ $$aBackground: Livestock-associated (LA)-MRSA of CC398 lineage is related to the pig environment, although it also colonizes/infects humans. Tetracycline resistance (TETR) is a phenotypic marker of LA-MRSA-CC398.
Objectives: To determine the prevalence and changing epidemiology of LA-MRSA-CC398 in seven Spanish hospitals (H1-H7) located in areas with different pig farming densities (PFDs) (extremely high, very high, medium, and very low: EH/VH/M/VL), and to identify other non-CC398-LA-MRSA clones.
Methods: MRSA-TETR isolates (n = 165) obtained from hospitals H1-H7 over 6 months in 2023 were characterized with respect to genetic lineages/antimicrobial resistance and virulence/immune evasion cluster of CC398 and non-CC398. Results were compared with a previous multicentre study from 2016.
Results: We identified 86/165 MRSA-TETR isolates (52.1%) as being MRSA-CC398. A significant difference in MRSA-CC398/MRSA prevalence was detected between hospitals located in EH-PFD areas (H1 and H2, > 25%) and the one in a VL-PFD area (H7, 0%). Prevalences in the range 6.4%-12.2% were found in hospitals in M- and VH-PFD areas (H3-H6). Fourteen spa-types were identified among MRSA-CC398 isolates, with t011/t034 predominating (68.3%), followed by t1451/t1255 (13%). All but two MRSA-CC398 isolates were scn-negative (human adaptation marker). Among the 79 non-CC398-MRSA-TETR isolates, CC5, CC1 and CC8 predominated (45.6%, scn-negative). The prevalences of the scn gene among non-CC398-MRSA-TETR isolates in hospitals of EH-, VH-, M- and VL-PFD areas were 77.8%, 50%, 18.75% and 0%, respectively (significant correlation, P < 0.05). This study shows that MRSA-CC398 isolates are prevalent in hospitals located in EH-PFD areas but absent in the hospital of the VL-PFD area. Moreover, an increase in both PFD and prevalence of MRSA-CC398/MRSA in hospitals H3-H6 was observed in the 2023 study compared with the 2016 study.
Conclusions: A significant increase and more genetic diversity of predominant lineages of CC398-MRSA-TETR were observed in hospitals located in M- to VH-PFD areas.
000156603 536__ $$9info:eu-repo/grantAgreement/ES/AEI/PID2022-139591OB-I00
000156603 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000156603 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000156603 700__ $$aLatorre-Fernández, Javier
000156603 700__ $$aEguizábal, Paula
000156603 700__ $$aBellés-Bellés, Alba
000156603 700__ $$aMormeneo-Bayo, Saray
000156603 700__ $$aAlonso, Carla Andrea
000156603 700__ $$aArregui, Irati
000156603 700__ $$aLópez-Calleja, Ana Isabel
000156603 700__ $$0(orcid)0000-0001-7294-245X$$aRezusta, Antonio
000156603 700__ $$0(orcid)0000-0002-9742-1463$$aSeral, Cristina$$uUniversidad de Zaragoza
000156603 700__ $$0(orcid)0000-0002-2519-701X$$aCastillo, Francisco Javier$$uUniversidad de Zaragoza
000156603 700__ $$aVilamala, Anna
000156603 700__ $$aNavarro, María
000156603 700__ $$aAspiroz, Carmen
000156603 700__ $$aCebollada, Rocío
000156603 700__ $$aCercenado, Emilia
000156603 700__ $$aZarazaga, Myriam
000156603 700__ $$aLozano, Carmen
000156603 700__ $$aTorres, Carmen
000156603 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología
000156603 773__ $$g(2025), [10 pp.]$$pJ. antimicrob. chemother.$$tThe Journal of antimicrobial chemotherapy$$x0305-7453
000156603 8564_ $$s546590$$uhttps://zaguan.unizar.es/record/156603/files/texto_completo.pdf$$yVersión publicada
000156603 8564_ $$s2776784$$uhttps://zaguan.unizar.es/record/156603/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000156603 909CO $$ooai:zaguan.unizar.es:156603$$particulos$$pdriver
000156603 951__ $$a2025-10-17-14:24:26
000156603 980__ $$aARTICLE