000156609 001__ 156609
000156609 005__ 20251017144634.0
000156609 0247_ $$2doi$$a10.1186/s12864-025-11610-0
000156609 0248_ $$2sideral$$a143876
000156609 037__ $$aART-2025-143876
000156609 041__ $$aeng
000156609 100__ $$aMontero-Pau, Javier
000156609 245__ $$aTemperature-induced variation in the transcriptome of maritime pine (Pinus pinaster Ait.) embryogenic masses modulates the phenotype of the derived plants
000156609 260__ $$c2025
000156609 5060_ $$aAccess copy available to the general public$$fUnrestricted
000156609 5203_ $$aA number of studies show that combining somatic embryogenesis with environmental stimuli can induce plant defenses against abiotic stresses, offering a complementary strategy in tree breeding programs. In a previous study, we found that increasing/decreasing the standard temperature of 23 ˚C by 5 ˚C during maritime pine (Pinus pinaster) somatic embryo maturation resulted in epitypes, as the derived plants showed altered phenotypes regarding leaf histology, proline content, photosynthetic rates, and hormone profiles, and that also differentially respond after a short-term heat stress. To elucidate the mechanisms underlying these altered phenotypes, we sequenced the transcriptome of embryonal-suspensor masses (EMs) from the three epitypes, identifying 812 differentially expressed genes (DEGs). Ten genes involved in epigenetic regulation were specifically up-regulated in EMs of the cold epitype. While some of these genes have been linked with somatic embryo maturation, the increased expression of three of these genes, histone deacetylases HDA9, a histone-lysine methyl-transferase (HKMT) and an Argonaute (AGO7), was found to be low temperature-induced epigenetic marks. Among the genes up-regulated in the EMs from the warm epitype, we studied those related to abiotic stress response and observed greater variation in genes involved in abscisic acid (ABA)-mediated response such as those encoding Ras GTPase-activating protein-binding (G3BP) proteins, an AAA-ATPase, and an aspartyl protease (APF2). We also found differential expression in genes encoding for RING-type E3 ubiquitin-transferases, and DNAJ and BAG chaperones. Additionally, the biosynthetic pathways of jasmonic acid, cytokinins and the diterpene pimaradiene were also altered in the warm epitype. However, the increased ABA and cytokinin content observed in the plants derived from this warm epitype cannot be fully explained by the EMs transcriptome profile. Conversely, in the cold epitype, we observed downregulation of genes encoding for an ABA receptor (PYL3), and a xyloglucan endotrans-glucosylase/hydrolase (XTH6). These findings support the hypothesis that the previously reported heat-adapted phenotype of plants derived from the cold epitype (characterized by a faster and higher proline increase, lower increases in ABA levels, no reduction in active cytokinins, and a better net photosynthesis rate recovery) could be attributed to low-temperature-induced epigenetic marks that were absent in the warm epitype.
000156609 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-112627RB-C31
000156609 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000156609 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000156609 700__ $$aPérez-Oliver, María Amparo
000156609 700__ $$0(orcid)0009-0002-2346-7431$$aRodríguez-Cuesta, Álvaro$$uUniversidad de Zaragoza
000156609 700__ $$aArrillaga, Isabel
000156609 700__ $$0(orcid)0000-0003-0582-5418$$aSales, Ester$$uUniversidad de Zaragoza
000156609 7102_ $$15011$$2705$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Producción Vegetal
000156609 773__ $$g26 (2025), 467 [17 pp.]$$pBMC genomics$$tBMC Genomics$$x1471-2164
000156609 787__ $$tBioProject PRJNA1077792$$whttps://dataview.ncbi.nlm.nih.gov/object/PRJNA1077792?reviewer=a0ou4ekoslho1g9qojfsdm1van
000156609 8564_ $$s2263188$$uhttps://zaguan.unizar.es/record/156609/files/texto_completo.pdf$$yVersión publicada
000156609 8564_ $$s2190386$$uhttps://zaguan.unizar.es/record/156609/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000156609 909CO $$ooai:zaguan.unizar.es:156609$$particulos$$pdriver
000156609 951__ $$a2025-10-17-14:27:57
000156609 980__ $$aARTICLE