000156621 001__ 156621
000156621 005__ 20251017144641.0
000156621 0247_ $$2doi$$a10.1186/s12870-025-06445-6
000156621 0248_ $$2sideral$$a143857
000156621 037__ $$aART-2025-143857
000156621 041__ $$aeng
000156621 100__ $$aBielsa, F. J.
000156621 245__ $$aComparative enzymatic browning transcriptome analysis of three apple cultivars unravels a conserved regulatory network related to stress responses
000156621 260__ $$c2025
000156621 5060_ $$aAccess copy available to the general public$$fUnrestricted
000156621 5203_ $$aEnzymatic browning (EB) endangers the adaptation of apple fruit cultivars to new markets, affecting organoleptic properties and producing economic losses. Polyphenol oxidases and polyphenol compounds play a key role in EB development in apple. However, the regulation of apple response to EB remains to be uncovered. In this study, three apple cultivars with different EB phenotypes ranging from low to high browning in apple pulp were used to study transcriptomic changes over time after fresh cutting (0, 30 and 60 min). This study allowed the identification of 1448 differentially expressed genes (DEGs), revealing both shared and genotype-specific responses, particularly in the affected metabolic pathways associated with EB. At 60 min (T60 vsT0), 77 DEGs were shared by all genotypes, suggesting a conserved regulatory network. This network included genes encoding for protein families such as calcium-binding proteins, heat-shock proteins, redox-responsive transcription factors, WRKY family transcription factors, zinc finger family proteins and disease resistance proteins among others. A co-expressed gene cluster, identified through Weighed Gene Co-Expression Network Analysis (WGCNA), was found to correlate with EB and included 323 genes enriched in several biological terms according to Gene Ontology analysis. Moreover, a more detailed analysis of identified WGCNA gene cluster regulatory sequences allowed the detection of cis-regulatory elements belonging to CAMTA, WRKY and WUSCHEL transcription factor families. The identification of these sequences alongside with an abundant and diverse amount of overexpressed transcription factors from various families (WRKY, ERF, GRAS, GATA, etc.) point out to a highly regulated stress-response that is strictly connected to innate plant immunity. These findings provide valuable insights into the molecular mechanism involved in apple fresh-cut browning and offer new potential targets for EB regulation.
000156621 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A12$$9info:eu-repo/grantAgreement/ES/MICINN-AEI/PID2019-108081RR-C21
000156621 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000156621 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000156621 700__ $$aGrimplet, J.
000156621 700__ $$aIrisarri, P.
000156621 700__ $$aMiranda, C.
000156621 700__ $$aErrea, P.
000156621 700__ $$0(orcid)0000-0002-4988-9467$$aPina, A.
000156621 773__ $$g25, 1 (2025), 25 pp.$$pBMC PLANT BIOLOGY$$tBMC PLANT BIOLOGY$$x1471-2229
000156621 8564_ $$s5301052$$uhttps://zaguan.unizar.es/record/156621/files/texto_completo.pdf$$yVersión publicada
000156621 8564_ $$s2114458$$uhttps://zaguan.unizar.es/record/156621/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000156621 909CO $$ooai:zaguan.unizar.es:156621$$particulos$$pdriver
000156621 951__ $$a2025-10-17-14:32:12
000156621 980__ $$aARTICLE