000156639 001__ 156639
000156639 005__ 20251030145435.0
000156639 0247_ $$2doi$$a10.1016/j.placenta.2025.02.018
000156639 0248_ $$2sideral$$a143905
000156639 037__ $$aART-2025-143905
000156639 041__ $$aeng
000156639 100__ $$aMedel-Martinez, Ana
000156639 245__ $$aThe placental transcriptomic profile determined by maternal COVID-19 disease encompasses alterations reminiscent of preeclampsia
000156639 260__ $$c2025
000156639 5060_ $$aAccess copy available to the general public$$fUnrestricted
000156639 5203_ $$aIntroduction. During initial stages of the pandemic, SARS-CoV-2 infection during pregnancy was related to adverse pregnancy outcomes and alterations in the placenta. Whether placental abnormalities in pregnant women with COVID-19 still persist afterwards remains poorly studied. Here, we determined whether the absence of obstetric complications after maternal COVID-19 disease, including preeclampsia, is accompanied by a complete return to normalcy in terms of placental physiology. Methods. Placental RNA was purified from placental samples from SARS-CoV-2 positive mothers taken either in 2022, when Omicron was the predominant variant of concern (termed Omicron) (n = 21); or from healthy pregnancies predating the pandemic (termed preCOVID-19). Our cohort included samples from pregnant women who got infected weeks and even months before term. We performed RNA-seq, identified differentially expressed genes and examined to which biological, biochemical and cellular pathways they belong, using gene set enrichment analysis. Results. We identified 71 differentially expressed genes (DEGs) (defined by p-value ≤0.05 and fold change (FC) of ≤ -2 or ≥2). The alterations identified in placentas delivered by mothers who suffered an episode of COVID-19 disease could be mainly attributed to pathways related to organogenesis, extracellular matrix organization and oxygen transport. These alterations were also detected after exclusion of gestational diabetes mellitus (GDM) samples. Although none of the samples were taken from cases of preeclampsia, several of the relevant DEGs have been previously reported as dysregulated in hypertensive disorders of pregnancy including preeclampsia. Discussion. We propose that maternal SARS-CoV-2 infection causes alterations in gene expression that are indicative of vascular defects in the placenta
000156639 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B46-20R$$9info:eu-repo/grantAgreement/ES/DGA/B46-23R$$9info:eu-repo/grantAgreement/ES/DGA/LMP228_21$$9info:eu-repo/grantAgreement/ES/ISCIII-FEDER/CM21-00045$$9nfo:eu-repo/grantAgreement/ES/ISCIII/FI21-00224$$9info:eu-repo/grantAgreement/ES/ISCIII/INT20-00033$$9info:eu-repo/grantAgreement/ES/ISCIII/JR19-00006
000156639 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000156639 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/submittedVersion
000156639 700__ $$aStrunk, Mark
000156639 700__ $$0(orcid)0000-0002-3312-7759$$aCebollada-Solanas, Alberto
000156639 700__ $$aPuente-Santamaría, Laura
000156639 700__ $$aGómez-Muñoz, Sonia$$uUniversidad de Zaragoza
000156639 700__ $$aFabre, Marta$$uUniversidad de Zaragoza
000156639 700__ $$0(orcid)0000-0001-6795-0367$$aPaules, Cristina$$uUniversidad de Zaragoza
000156639 700__ $$0(orcid)0000-0002-8476-9161$$aOros, Daniel$$uUniversidad de Zaragoza
000156639 700__ $$aSchoorlemmer, Jon
000156639 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000156639 7102_ $$11013$$2645$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Obstetricia y Ginecología
000156639 7102_ $$11012$$2X$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Técnica. Lab. y Talleres
000156639 773__ $$g165 (2025), 127-135$$pPlacenta$$tPLACENTA$$x0143-4004
000156639 8564_ $$s1064003$$uhttps://zaguan.unizar.es/record/156639/files/texto_completo.pdf$$yPreprint
000156639 8564_ $$s1530088$$uhttps://zaguan.unizar.es/record/156639/files/texto_completo.jpg?subformat=icon$$xicon$$yPreprint
000156639 909CO $$ooai:zaguan.unizar.es:156639$$particulos$$pdriver
000156639 951__ $$a2025-10-30-14:52:16
000156639 980__ $$aARTICLE