156674 20251017144613.0 doi 10.1002/adfm.202420106 sideral 143959 ART-2025-143959 eng Iglesias-García, Olalla Biologically-Inspired Melt Electrowriting for the Generationof Highly Biomimetic Functional Myocardium 2025 Access copy available to the general public Unrestricted In the heart, the specific 3D structure of myocardial layers produces an efficient ejection of blood. When myocardial infarction strikes, this architecture is disrupted, adding a disarranged contraction to the decreased availability of pumping units (cardiomyocytes, CMs). In this work, the alignment of cardiac fibers in a large animal model (pig) is characterized and employ melt electrowriting (MEW) to fabricate a bio-inspired scaffold with diamond-shaped pores. Using human-induced pluripotent stem cell-derived CMs and cardiac fibroblasts, human cardiac tissues with a biomimetic in-plane contraction are generated. MEW-diamond tissues beat macroscopically for over 1 month, with significantly faster kinetics, increased force, and higher conduction velocity than those based on square or rectangular pores. The diamond design induces a specific hiPSC-CM alignment resulting in the observed in-plane contraction. Transcriptomic analysis using bulk RNA-seq reveals diamond-MEW tissues present features of maturation as compared to traditional 2D cultures. Finally, the bio-inspired cardiac tissues are employed to treat an infarction model in athymic rats, showing a significant benefit on systolic function and remodeling, tied to the presence of large grafts of human cells remuscularizing the ventricular wall. All in all, it is demonstrated that the new design generates superior human cardiac tissues with therapeutic capacity. info:eu-repo/grantAgreement/ES/DGA/T39-23R info:eu-repo/grantAgreement/EC/H2020/874827/EU/Computational biomechanics and bioengineering 3D printing to develop a personalized regenerative biological ventricular assist device to provide lasting functional support to damaged hearts/BRAV3 This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 874827-BRAV3 info:eu-repo/grantAgreement/ES/ISCIII/CD22-00027 info:eu-repo/grantAgreement/ES/MCIN/INVESTTRA_PID2022-142807OA-I00 info:eu-repo/grantAgreement/ES/MCIN/PLEC2021-008127 info:eu-repo/grantAgreement/ES/MCIN/VOLVAD_PID2022-142562OB-I00 info:eu-repo/grantAgreement/ES/MICINN/PID2022-140556OB-I00 info:eu-repo/grantAgreement/EUR/MICINN/TED2021-130459B-I00 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Flandes-Iparraguirre, María Montero-Calle, Pilar Rosales, Ricardo M. Ullate-Agote, Asier Sánchez-Bueno, Andrea Larequi, Eduardo Anaut-Lusar, Ilazki Laita, Nicolás (orcid)0000-0001-8946-4829 Oliván-Viguera, Aida (orcid)0000-0001-5348-924X Iglesias, Elena Abizanda, Gloria San Martín-Úriz, Patxi Aguirre-Ruiz, Paula Aranguren, Xabier L. García de Yébenes, Manuel Gavira, Juan José Martínez, Miguel Ángel Peña, Estefanía Universidad de Zaragoza (orcid)0000-0002-0664-5024 Doblaré, Manuel Universidad de Zaragoza (orcid)0000-0001-8741-6452 de-Juan-Pardo, Elena M. Pueyo, Esther Universidad de Zaragoza (orcid)0000-0002-1960-407X Prosper, Felipe Mazo Vega, Manuel M. 5008 800 Universidad de Zaragoza Dpto. Ingeniería Electrón.Com. Área Teoría Señal y Comunicac. 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. (2025), 2420106 [20 pp.] Adv. funct. mater. Advanced Functional Materials 1616-301X 4439329 http://zaguan.unizar.es/record/156674/files/texto_completo.pdf Versión publicada 2706181 http://zaguan.unizar.es/record/156674/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:156674 articulos driver 2025-10-17-14:18:16 ARTICLE