000160792 001__ 160792
000160792 005__ 20251017144556.0
000160792 0247_ $$2doi$$a10.1128/spectrum.03111-24
000160792 0248_ $$2sideral$$a143971
000160792 037__ $$aART-2025-143971
000160792 041__ $$aeng
000160792 100__ $$0(orcid)0009-0008-7807-0400$$aDehesa-García, Blanca
000160792 245__ $$aEnhancing Candida auris diagnosis: a comprehensive evaluation of VIASURE Candida auris real-time PCR detection kit for rapid diagnostic responses
000160792 260__ $$c2025
000160792 5060_ $$aAccess copy available to the general public$$fUnrestricted
000160792 5203_ $$aCandida auris, a yeast that is highly resistant to antifungal treatments, represents a significant healthcare concern due to its rapid spread through surface contamination and patient colonization. A recent outbreak at the Miguel Servet University Hospital in Zaragoza, Spain, highlighted the urgent need for rapid diagnostic responses. The current 4-day duration for microbiological culture results hinders timely patient isolation and transmission prevention. This study aims to evaluate the VIASURE Candida auris Real-Time PCR Detection Kit (Certest Biotec) for diagnostic performance and analytical specificity compared to the standard methodology based on culture and MALDI-TOF MS characterization. From August 2023 to December 2023, the Microbiology Service of the Miguel Servet University Hospital processed 816 diagnostic samples. Accuracy testing resulted in adequate clinical validation values, as presented here: sensitivity, 0.98 (95% confidence interval [CI], 0.90 to 1); specificity, 1 (95% CI, 0.99 to 1); positive predictive value, 0.98 (95% CI, 0.90 to 1); and negative predictive value, 1 (95% CI, 0.99 to 1). The implementation of this molecular biology test in the laboratory promises to improve diagnostic efficiency by allowing rapid patient isolation, initiation of appropriate treatment, and effective isolation measures.
000160792 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000160792 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000160792 700__ $$0(orcid)0000-0003-2742-8827$$aAlonso, Henar
000160792 700__ $$0(orcid)0000-0001-5750-3643$$aPeris, María Paz$$uUniversidad de Zaragoza
000160792 700__ $$aFortuño, Blanca
000160792 700__ $$aAbad, Pilar
000160792 700__ $$0(orcid)0000-0001-7294-245X$$aRezusta, Antonio
000160792 700__ $$aMilagro, Ana
000160792 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000160792 773__ $$g(2025), e03111-24 [10 pp.]$$pMicrobiol. spectr.$$tMicrobiology Spectrum$$x2165-0497
000160792 8564_ $$s293994$$uhttps://zaguan.unizar.es/record/160792/files/texto_completo.pdf$$yVersión publicada
000160792 8564_ $$s2270946$$uhttps://zaguan.unizar.es/record/160792/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000160792 909CO $$ooai:zaguan.unizar.es:160792$$particulos$$pdriver
000160792 951__ $$a2025-10-17-14:13:12
000160792 980__ $$aARTICLE