000161118 001__ 161118
000161118 005__ 20251017144645.0
000161118 0247_ $$2doi$$a10.1016/j.apsoil.2025.106216
000161118 0248_ $$2sideral$$a144258
000161118 037__ $$aART-2025-144258
000161118 041__ $$aeng
000161118 100__ $$aHernández Maqueda, R.
000161118 245__ $$aInfluence of green manuring on soil properties, abundance and expression of key denitrification genes in a greenhouse Anthrosol
000161118 260__ $$c2025
000161118 5060_ $$aAccess copy available to the general public$$fUnrestricted
000161118 5203_ $$aIntercropping with green manure is a valuable agronomic practice for promoting sustainability agricultural systems. The aim of this study was to evaluate the effects of green manure (GM) – based on a mixture of vetch (Vicia sativa L.), forage turnip (Brassica rapa L. subsp. rapa), and oats (Avena sativa L.) – on the physicochemical properties of a greenhouse Anthrosol and its impact on the abundance and expression of denitrifying genes (nirK, nirS, and nosZ (clade I and II)) using real-time PCR. The cultivated crop throughout the three-year experiment was eggplant (Solanum melongena L. subs. Telma) fertilized with manure and crop residues from the previous growing season. A linear mixed model (LMM) was used to assess the effect of GM on both the physicochemical variables and the target-gene and transcript copy number in the soil. After three years of GM coverage, moderate changes in soil physicochemical properties were observed, with the exception of a significant decrease in soil temperature (<6 %) and ammonium ion concentration (<14 %) with GM application. Gene copy numbers remained largely unchanged between treatments, however transcripts levels decreased for all target genes under GM, with particularly pronounced reduction for nirK (p < 0.0001) and nosZ II (p < 0.05), suggesting partial suppression of gene transcription within the denitrification pathway. In addition, potassium (K+) and soil moisture were found to correlate with DNA and RNA abundances, indicating a complex interaction between salinity and soil moisture in the regulation of the denitrification process.
000161118 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000161118 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000161118 700__ $$aBallesteros, I.
000161118 700__ $$0(orcid)0000-0001-5859-3361$$aAguirre, A. J.$$uUniversidad de Zaragoza
000161118 700__ $$aMeca, D.
000161118 700__ $$aLinacero, R.
000161118 700__ $$aMoral, F. del
000161118 7102_ $$15002$$2720$$aUniversidad de Zaragoza$$bDpto. Ingeniería Diseño Fabri.$$cÁrea Proyectos de Ingeniería
000161118 773__ $$g212 (2025), 106216 [9 p.]$$pAgric., ecosyst. environ., Appl. soil ecol.$$tAPPLIED SOIL ECOLOGY$$x0929-1393
000161118 8564_ $$s2710082$$uhttps://zaguan.unizar.es/record/161118/files/texto_completo.pdf$$yVersión publicada
000161118 8564_ $$s2454044$$uhttps://zaguan.unizar.es/record/161118/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000161118 909CO $$ooai:zaguan.unizar.es:161118$$particulos$$pdriver
000161118 951__ $$a2025-10-17-14:33:28
000161118 980__ $$aARTICLE