161118 20251017144645.0 doi 10.1016/j.apsoil.2025.106216 sideral 144258 ART-2025-144258 eng Hernández Maqueda, R. Influence of green manuring on soil properties, abundance and expression of key denitrification genes in a greenhouse Anthrosol 2025 Access copy available to the general public Unrestricted Intercropping with green manure is a valuable agronomic practice for promoting sustainability agricultural systems. The aim of this study was to evaluate the effects of green manure (GM) – based on a mixture of vetch (Vicia sativa L.), forage turnip (Brassica rapa L. subsp. rapa), and oats (Avena sativa L.) – on the physicochemical properties of a greenhouse Anthrosol and its impact on the abundance and expression of denitrifying genes (nirK, nirS, and nosZ (clade I and II)) using real-time PCR. The cultivated crop throughout the three-year experiment was eggplant (Solanum melongena L. subs. Telma) fertilized with manure and crop residues from the previous growing season. A linear mixed model (LMM) was used to assess the effect of GM on both the physicochemical variables and the target-gene and transcript copy number in the soil. After three years of GM coverage, moderate changes in soil physicochemical properties were observed, with the exception of a significant decrease in soil temperature (<6 %) and ammonium ion concentration (<14 %) with GM application. Gene copy numbers remained largely unchanged between treatments, however transcripts levels decreased for all target genes under GM, with particularly pronounced reduction for nirK (p < 0.0001) and nosZ II (p < 0.05), suggesting partial suppression of gene transcription within the denitrification pathway. In addition, potassium (K+) and soil moisture were found to correlate with DNA and RNA abundances, indicating a complex interaction between salinity and soil moisture in the regulation of the denitrification process. info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Ballesteros, I. Aguirre, A. J. Universidad de Zaragoza (orcid)0000-0001-5859-3361 Meca, D. Linacero, R. Moral, F. del 5002 720 Universidad de Zaragoza Dpto. Ingeniería Diseño Fabri. Área Proyectos de Ingeniería 212 (2025), 106216 [9 p.] Agric., ecosyst. environ., Appl. soil ecol. APPLIED SOIL ECOLOGY 0929-1393 2710082 http://zaguan.unizar.es/record/161118/files/texto_completo.pdf Versión publicada 2454044 http://zaguan.unizar.es/record/161118/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:161118 articulos driver 2025-10-17-14:33:28 ARTICLE