000161690 001__ 161690
000161690 005__ 20251017144556.0
000161690 0247_ $$2doi$$a10.1016/j.foodcont.2025.111475
000161690 0248_ $$2sideral$$a144360
000161690 037__ $$aART-2025-144360
000161690 041__ $$aeng
000161690 100__ $$0(orcid)0000-0002-5309-7796$$aAguerri, Laura$$uUniversidad de Zaragoza
000161690 245__ $$aA novel biodegradable active packaging based on PHB/PCL for controlling Listeria monocytogenes in fresh salmon
000161690 260__ $$c2025
000161690 5060_ $$aAccess copy available to the general public$$fUnrestricted
000161690 5203_ $$aOur study was focused on developing a novel active packaging material with antibacterial properties to ensure food safety, especially against Listeria monocytogenes. For this purpose, blends formed by a mixture of biodegradable polymers, polyhydroxybutyrate (PHB) and polycaprolactone (PCL), and different ε-poly-L-lysine (EPL) concentrations, were produced by extrusion. Then, active laminates were prepared by thermoforming and characterized by TGA, DSC and FT-IR. Besides, an EPL release assay into food simulants was performed and a sustained release was observed. Laminates activity was evaluated against Listeria monocytogenes and Salmonella enterica, both in liquid and solid media, where they exhibited high antibacterial activities, especially in the case of Listeria. To further study whether the developed materials maintained their active properties when in contact with contaminated food, a challenge test was performed on Listeria monocytogenes-inoculated fresh salmon. For that purpose, tray packaging prototypes containing 3 and 5 % EPL were produced by injection moulding and used to store salmon for 12 days. Microbial counts (Listeria monocytogenes, Lactic Acid Bacteria, Pseudomonas spp. and Total Viable Counts), and total volatile basic nitrogen (TVB-N) were determined over time. Results showed that active tray prototypes did not affect the quality of the stored salmon in terms of TVB-N. Moreover, they yielded significant reductions on microbial counts for all bacterial groups when compared to control samples. Thus, biodegradable antimicrobial developed trays effectively controlled Listeria monocytogenes and other spoilage bacteria present in fresh salmon.
000161690 536__ $$9info:eu-repo/grantAgreement/ES/DGA/T53-23R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-128089OB-I00
000161690 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000161690 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000161690 700__ $$aLeonardi, Frédéric
000161690 700__ $$0(orcid)0000-0001-7143-8905$$aAlmeida E Silva, Filomena Augusta
000161690 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000161690 773__ $$g178 (2025), 111475 [11 pp.]$$pFood control$$tFood Control$$x0956-7135
000161690 8564_ $$s7881348$$uhttps://zaguan.unizar.es/record/161690/files/texto_completo.pdf$$yVersión publicada
000161690 8564_ $$s2567119$$uhttps://zaguan.unizar.es/record/161690/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000161690 909CO $$ooai:zaguan.unizar.es:161690$$particulos$$pdriver
000161690 951__ $$a2025-10-17-14:13:20
000161690 980__ $$aARTICLE