000161691 001__ 161691
000161691 005__ 20251017144601.0
000161691 0247_ $$2doi$$a10.1021/acs.analchem.5c00752
000161691 0248_ $$2sideral$$a144352
000161691 037__ $$aART-2025-144352
000161691 041__ $$aeng
000161691 100__ $$aZhang, Yao-Yao
000161691 245__ $$aImproved ESI-MS Sensitivity via an Imidazolium Tag (DAPMI-ITag) for Precise Sialic Acid Detection in Human Serum and CMAH-Null Mouse Tissues
000161691 260__ $$c2025
000161691 5060_ $$aAccess copy available to the general public$$fUnrestricted
000161691 5203_ $$aSialic acids (Sias), consisting primarily of N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), play crucial roles in many biological processes. The detection and quantification of Sias are essential for understanding their roles in health and disease progression. Although numerous techniques have been developed to enhance the specificity and sensitivity of Sias analysis, traditional methods such as derivatization with fluorescent tags coupled with HPLC-MS analysis often suffer from low limits of detection, limiting the quantification of Sias in trace samples. Here, we introduce DAPMI, a novel imidazolium-based ITag for sensitive Sia detection. We demonstrate its utility in the detection and quantification of Sia composition in human serum, and in different tissues from CMAH (cytidine monophosphate-N-acetylneuraminic acid hydroxylase) knockout mice, using ESI-MS analysis and with a limit of detection (LOD) down to the low fmol range. The results showed that both Neu5Ac and Neu5Gc were present in varying proportions in wild-type mice and CMAH heterogeneous mice. Trace amounts of Neu5Gc were also detected in the tissues of CMAH null homogeneous mice (CMAH–/−) and in human blood serum using ESI-ToF-MS, suggesting its presence may be linked to dietary intake of Neu5Gc-containing foods, as Neu5Gc cannot be synthesized endogenously in CMAH–/– mice, and in humans. The DAPMI-ITag and the labeling technology developed in this study significantly improve the sensitivity of Sias detection compared to conventional tags such as o-phenylenediamine (OPD), and provide a new chemical tool for the exploration of Sias’ biological roles
000161691 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000161691 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000161691 700__ $$aHu, Zi-Xuan
000161691 700__ $$aZhang, Si-Yu
000161691 700__ $$aLiu, Li
000161691 700__ $$aGalan, M. Carmen
000161691 700__ $$aVoglmeir, Josef
000161691 700__ $$0(orcid)0000-0002-2855-4801$$aGhirardello, Mattia$$uUniversidad de Zaragoza
000161691 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000161691 773__ $$g(2025), [8 pp.]$$pAnal. chem.$$tANALYTICAL CHEMISTRY$$x0003-2700
000161691 8564_ $$s3940335$$uhttps://zaguan.unizar.es/record/161691/files/texto_completo.pdf$$yVersión publicada
000161691 8564_ $$s3056169$$uhttps://zaguan.unizar.es/record/161691/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000161691 909CO $$ooai:zaguan.unizar.es:161691$$particulos$$pdriver
000161691 951__ $$a2025-10-17-14:14:10
000161691 980__ $$aARTICLE