000161975 001__ 161975
000161975 005__ 20251017144600.0
000161975 0247_ $$2doi$$a10.1039/d5bm00387c
000161975 0248_ $$2sideral$$a144284
000161975 037__ $$aART-2025-144284
000161975 041__ $$aeng
000161975 100__ $$aAvasthi, Ashish
000161975 245__ $$aProtein corona as the key factor governing the in vivo fate of magnetic nanoparticles
000161975 260__ $$c2025
000161975 5060_ $$aAccess copy available to the general public$$fUnrestricted
000161975 5203_ $$aAdvancements in the field of nanotechnology have opened a myriad of avenues for diverse applications. One such avenue is the role of nanoparticles (NPs) in the healthcare sector, whether it is drug targeting, drug delivery, or imaging, offering unprecedented prospects for improving targeted interventions along with minimal toxicity. Meanwhile, the intricate interplay between the characteristics of NPs and the ensuing biological cross-talk has engendered profound interest among scientists. Amidst the determinants shaping the behavior of NPs within the biological milieu, the biodistribution and pharmacokinetics of the NPs stand as pivotal factors intricately intertwined with their core size, hydrodynamic diameter (HD), coating ligands, as well as the proteins they interact with, forming the protein corona. This article compiles and analyzes the data to decipher the factors determining the fate of NPs in vivo. For this purpose, two IONPs with differing core sizes (≈4 nm and ≈8 nm) but coated with the same gallol-PEG ligand (GA-PEG3000-OH) and possessing very similar HDs (≈35 nm) as well as relaxivity (≈100 mM−1 s−1) were selected. Following physicochemical characterization, both protein coronas were thoroughly analyzed, revealing differences in both the composition and the relative abundance. Later, after determining the negligible cytotoxicity of both NPs, they were intravenously injected into Balb/c mice to evaluate their in vivo biodistribution and pharmacokinetics using MRI. Additionally, biodistribution was further investigated ex vivo by quantitative magnetic analysis of blood and tissues, alongside histological evaluation. Our results evidenced that the protein corona, rather than core size or hydrodynamic diameter, is the determining factor governing the in vivo fate of magnetic NPs.
000161975 540__ $$9info:eu-repo/semantics/embargoedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000161975 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000161975 700__ $$0(orcid)0000-0002-0970-1917$$aFernández-Afonso, Yilian
000161975 700__ $$0(orcid)0000-0003-2366-3598$$aGutiérrez, Lucía
000161975 700__ $$0(orcid)0000-0003-1081-8482$$aFuente, Jesús M. de la
000161975 700__ $$aPernía Leal, Manuel
000161975 700__ $$aCaro, Carlos
000161975 700__ $$aGarcia-Martin, Maria Luisa
000161975 773__ $$g13 (2025), 3840-3852$$pBiomater. sci.$$tBiomaterials Science$$x2047-4830
000161975 8564_ $$s5126080$$uhttps://zaguan.unizar.es/record/161975/files/texto_completo.pdf$$yPostprint$$zinfo:eu-repo/date/embargoEnd/2026-05-29
000161975 8564_ $$s3022417$$uhttps://zaguan.unizar.es/record/161975/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint$$zinfo:eu-repo/date/embargoEnd/2026-05-29
000161975 909CO $$ooai:zaguan.unizar.es:161975$$particulos$$pdriver
000161975 951__ $$a2025-10-17-14:14:09
000161975 980__ $$aARTICLE