000161979 001__ 161979
000161979 005__ 20251017144603.0
000161979 0247_ $$2doi$$a10.1084/jem.20241591
000161979 0248_ $$2sideral$$a144638
000161979 037__ $$aART-2025-144638
000161979 041__ $$aeng
000161979 100__ $$aJohansson, Åsa
000161979 245__ $$aNeonatal microbiota colonization primes maturation of goblet cell–mediated protection in the pre-weaning colon
000161979 260__ $$c2025
000161979 5060_ $$aAccess copy available to the general public$$fUnrestricted
000161979 5203_ $$aRegulated host–microbe interactions are a critical aspect of lifelong health. Colonic goblet cells protect from microorganisms via the generation of a mucus barrier structure. Bacteria-sensing sentinel goblet cells provide secondary protection by orchestrating mucus secretion when microbes breach the mucus barrier. Mucus deficiencies in germ-free mice implicate a role for the microbiota in programming barrier generation, but its natural ontogeny remains undefined. We now investigate the mucus barrier and sentinel goblet cell development in relation to postnatal colonization. Combined in vivo and ex vivo analyses demonstrate rapid and sequential microbiota-dependent development of these primary and secondary goblet cell protective functions, with dynamic changes in mucus processing dependent on innate immune signaling via MyD88 and development of functional sentinel goblet cells dependent on the NADPH/dual oxidase family member Duox2. Our findings identify new mechanisms of microbiota–goblet cell regulatory interaction and highlight the critical importance of the pre-weaning period for the normal development of protective systems that are key legislators of host–microbiota interaction.
000161979 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000161979 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000161979 700__ $$aVenkita Subramani, Mahadevan
000161979 700__ $$aYilmaz, Bahtiyar
000161979 700__ $$aNyström, Elisabeth E.L.
000161979 700__ $$0(orcid)0000-0001-5573-6144$$aLayunta, Elena$$uUniversidad de Zaragoza
000161979 700__ $$aArike, Liisa
000161979 700__ $$aSommer, Felix
000161979 700__ $$aRosenstiel, Philip
000161979 700__ $$aVereecke, Lars
000161979 700__ $$aMannerås-Holm, Louise
000161979 700__ $$aWullaert, Andy
000161979 700__ $$aPelaseyed, Thaher
000161979 700__ $$aJohansson, Malin E.V.
000161979 700__ $$aBirchenough, George M.H.
000161979 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000161979 773__ $$g222, 8 (2025), [27 pp.]$$pJ. exp. med.$$tJOURNAL OF EXPERIMENTAL MEDICINE$$x0022-1007
000161979 8564_ $$s8522554$$uhttps://zaguan.unizar.es/record/161979/files/texto_completo.pdf$$yVersión publicada
000161979 8564_ $$s3085774$$uhttps://zaguan.unizar.es/record/161979/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000161979 909CO $$ooai:zaguan.unizar.es:161979$$particulos$$pdriver
000161979 951__ $$a2025-10-17-14:14:37
000161979 980__ $$aARTICLE