161979 20251017144603.0 doi 10.1084/jem.20241591 sideral 144638 ART-2025-144638 eng Johansson, Åsa Neonatal microbiota colonization primes maturation of goblet cell–mediated protection in the pre-weaning colon 2025 Access copy available to the general public Unrestricted Regulated host–microbe interactions are a critical aspect of lifelong health. Colonic goblet cells protect from microorganisms via the generation of a mucus barrier structure. Bacteria-sensing sentinel goblet cells provide secondary protection by orchestrating mucus secretion when microbes breach the mucus barrier. Mucus deficiencies in germ-free mice implicate a role for the microbiota in programming barrier generation, but its natural ontogeny remains undefined. We now investigate the mucus barrier and sentinel goblet cell development in relation to postnatal colonization. Combined in vivo and ex vivo analyses demonstrate rapid and sequential microbiota-dependent development of these primary and secondary goblet cell protective functions, with dynamic changes in mucus processing dependent on innate immune signaling via MyD88 and development of functional sentinel goblet cells dependent on the NADPH/dual oxidase family member Duox2. Our findings identify new mechanisms of microbiota–goblet cell regulatory interaction and highlight the critical importance of the pre-weaning period for the normal development of protective systems that are key legislators of host–microbiota interaction. info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Venkita Subramani, Mahadevan Yilmaz, Bahtiyar Nyström, Elisabeth E.L. Layunta, Elena Universidad de Zaragoza (orcid)0000-0001-5573-6144 Arike, Liisa Sommer, Felix Rosenstiel, Philip Vereecke, Lars Mannerås-Holm, Louise Wullaert, Andy Pelaseyed, Thaher Johansson, Malin E.V. Birchenough, George M.H. 1001 025 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Anatom.Anatom.Patológ.Com 222, 8 (2025), [27 pp.] J. exp. med. JOURNAL OF EXPERIMENTAL MEDICINE 0022-1007 8522554 http://zaguan.unizar.es/record/161979/files/texto_completo.pdf Versión publicada 3085774 http://zaguan.unizar.es/record/161979/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:161979 articulos driver 2025-10-17-14:14:37 ARTICLE