000162022 001__ 162022 000162022 005__ 20251017144619.0 000162022 0247_ $$2doi$$a10.1167/tvst.14.6.24 000162022 0248_ $$2sideral$$a144659 000162022 037__ $$aART-2025-144659 000162022 041__ $$aeng 000162022 100__ $$aRamos Arizcuren, Ana M.$$uUniversidad de Zaragoza 000162022 245__ $$aA Method for Visualizing Corneal Dynamics Through Pixel Intensity Tracking 000162022 260__ $$c2025 000162022 5060_ $$aAccess copy available to the general public$$fUnrestricted 000162022 5203_ $$aPurpose: To develop a methodology for analyzing dynamic corneal images using pixel intensity tracking and hue, saturation, and value (HSV) color space transformation, aiming to quantify and visually interpret corneal responsiveness to mechanical stimulation. Methods: This study utilized the OCULUS Corvis ST device to obtain dynamic corneal images from two subject groups (young and older individuals). To ensure accurate pixel tracking, segmentation and registration techniques were implemented. To counteract potential biases due to alterations in curvature and eye retraction caused by the air-puff mechanical stimulus, the last 30 frames available post-stimulus were used for analysis. Pixel intensity tracking calculations were then performed in the frequency domain to compute H, S, and V values for each pixel within the overall region of interest. Additionally, corneal biomechanical parameters from the Corvis ST were extracted to examine their relationship with HSV values. Results: The HSV transformation provided a quantitative visual interpretation of corneal dynamics, with reddish vibrant areas indicating higher responsiveness to mechanical stimulation and dull blue areas indicating lower responsiveness. Additionally, significant differences were found between the young and older groups in each of the three HSV channels (all P < 0.001). Four biomechanical parameters showed statistically significant differences between groups (P < 0.05), but no statistically significant correlation with HSV values was found. Conclusions: The HSV methodology effectively visualizes and quantifies corneal responsiveness to mechanical stimulation, offering a new approach for assessing corneal dynamics. Translational Relevance: HSV color space analysis has the potential to enhance the diagnosis and management of conditions in which corneal dynamics are affected. 000162022 536__ $$9info:eu-repo/grantAgreement/EC/HORIZON EUROPE/101162733/EU/Visual Impairment Screening using Images from Ophthalmology and Novel pathways for Structural Analysis and Fast Evaluation/VISIONSAFE$$9info:eu-repo/grantAgreement/EUR/MICINN/TED2021-130723A-I00 000162022 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 000162022 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000162022 700__ $$0(orcid)0000-0001-5186-1837$$aConsejo, Alejandra$$uUniversidad de Zaragoza 000162022 7102_ $$12002$$2385$$aUniversidad de Zaragoza$$bDpto. Física Aplicada$$cÁrea Física Aplicada 000162022 773__ $$g14, 24 (2025), [12 pp.]$$pTransl. vis. sci. technol.$$tTranslational Vision Science and Technology$$x2164-2591 000162022 8564_ $$s2869362$$uhttps://zaguan.unizar.es/record/162022/files/texto_completo.pdf$$yVersión publicada 000162022 8564_ $$s2793979$$uhttps://zaguan.unizar.es/record/162022/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000162022 909CO $$ooai:zaguan.unizar.es:162022$$particulos$$pdriver 000162022 951__ $$a2025-10-17-14:21:03 000162022 980__ $$aARTICLE