000162105 001__ 162105
000162105 005__ 20251009133841.0
000162105 0247_ $$2doi$$a10.1080/01652176.2025.2532396
000162105 0248_ $$2sideral$$a144730
000162105 037__ $$aART-2025-144730
000162105 041__ $$aeng
000162105 100__ $$aMarteles, Diana$$uUniversidad de Zaragoza
000162105 245__ $$aIs long-term serum preservation suitable for research studies? Effect of time and temperature on the measurement of anti-Leishmania antibodies in canine sera samples
000162105 260__ $$c2025
000162105 5060_ $$aAccess copy available to the general public$$fUnrestricted
000162105 5203_ $$aThe stability of immunoglobulin G (IgG) antibodies is critical for diagnostic and research applications in veterinary medicine. This study evaluated the long-term stability of anti-Leishmania infantum IgG in canine serum samples under different storage conditions (−20 °C and −80 °C) over 2.5 years. Fifty-six serum samples were classified based on antibody concentration into low, medium, and high positive groups using an in-house enzyme-linked immunosorbent assay. Each sample was divided into aliquots and analyzed after different storage times (6 months, 1 year, 1.5, and 2.5 years). No statistically significant differences were observed in IgG concentrations across storage durations or between storage temperatures. Median antibody levels remained consistent, with minor variations attributed to assay-related variability. Correlation analyses showed strong agreement between initial and final measurements (R2 = 0.859 at −20 °C, R2 = 0.957 at −80 °C). The study underscores the suitability of −20 °C and −80 °C storage for preserving anti-Leishmania antibodies, providing valuable insights for serological diagnostics and research in veterinary science. Proper sample handling and aliquoting are recommended to maintain antibody integrity in routine diagnostics and long-term studies.
000162105 536__ $$9info:eu-repo/grantAgreement/ES/DGA/AR15-23R
000162105 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000162105 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000162105 700__ $$0(orcid)0000-0001-5816-6167$$aLebrero, María Eugenia$$uUniversidad de Zaragoza
000162105 700__ $$0(orcid)0000-0001-6209-4282$$aVillanueva-Saz, Sergio$$uUniversidad de Zaragoza
000162105 700__ $$0(orcid)0009-0005-0336-7582$$aEsteban Sanz, Clara$$uUniversidad de Zaragoza
000162105 700__ $$aMartín, Víctor
000162105 700__ $$0(orcid)0000-0002-2557-4890$$aFernández, Antonio$$uUniversidad de Zaragoza
000162105 700__ $$0(orcid)0009-0003-9757-3840$$aQuilez, Pablo$$uUniversidad de Zaragoza
000162105 700__ $$0(orcid)0000-0003-2957-1379$$aVerde, Maite$$uUniversidad de Zaragoza
000162105 700__ $$aGalan-Malo, Patricia$$uUniversidad de Zaragoza
000162105 700__ $$0(orcid)0000-0003-2555-8425$$aPérez, M. Dolores$$uUniversidad de Zaragoza
000162105 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000162105 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000162105 773__ $$g45, 1 (2025), 11-20$$pVet. q.$$tVETERINARY QUARTERLY$$x0165-2176
000162105 8564_ $$s2712721$$uhttps://zaguan.unizar.es/record/162105/files/texto_completo.pdf$$yVersión publicada
000162105 8564_ $$s1349758$$uhttps://zaguan.unizar.es/record/162105/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000162105 909CO $$ooai:zaguan.unizar.es:162105$$particulos$$pdriver
000162105 951__ $$a2025-10-09-13:25:56
000162105 980__ $$aARTICLE