162105 20251009133841.0 doi 10.1080/01652176.2025.2532396 sideral 144730 ART-2025-144730 eng Marteles, Diana Universidad de Zaragoza Is long-term serum preservation suitable for research studies? Effect of time and temperature on the measurement of anti-Leishmania antibodies in canine sera samples 2025 Access copy available to the general public Unrestricted The stability of immunoglobulin G (IgG) antibodies is critical for diagnostic and research applications in veterinary medicine. This study evaluated the long-term stability of anti-Leishmania infantum IgG in canine serum samples under different storage conditions (−20 °C and −80 °C) over 2.5 years. Fifty-six serum samples were classified based on antibody concentration into low, medium, and high positive groups using an in-house enzyme-linked immunosorbent assay. Each sample was divided into aliquots and analyzed after different storage times (6 months, 1 year, 1.5, and 2.5 years). No statistically significant differences were observed in IgG concentrations across storage durations or between storage temperatures. Median antibody levels remained consistent, with minor variations attributed to assay-related variability. Correlation analyses showed strong agreement between initial and final measurements (R2 = 0.859 at −20 °C, R2 = 0.957 at −80 °C). The study underscores the suitability of −20 °C and −80 °C storage for preserving anti-Leishmania antibodies, providing valuable insights for serological diagnostics and research in veterinary science. Proper sample handling and aliquoting are recommended to maintain antibody integrity in routine diagnostics and long-term studies. info:eu-repo/grantAgreement/ES/DGA/AR15-23R info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Lebrero, María Eugenia Universidad de Zaragoza (orcid)0000-0001-5816-6167 Villanueva-Saz, Sergio Universidad de Zaragoza (orcid)0000-0001-6209-4282 Esteban Sanz, Clara Universidad de Zaragoza (orcid)0009-0005-0336-7582 Martín, Víctor Fernández, Antonio Universidad de Zaragoza (orcid)0000-0002-2557-4890 Quilez, Pablo Universidad de Zaragoza (orcid)0009-0003-9757-3840 Verde, Maite Universidad de Zaragoza (orcid)0000-0003-2957-1379 Galan-Malo, Patricia Universidad de Zaragoza Pérez, M. Dolores Universidad de Zaragoza (orcid)0000-0003-2555-8425 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 45, 1 (2025), 11-20 Vet. q. VETERINARY QUARTERLY 0165-2176 2712721 http://zaguan.unizar.es/record/162105/files/texto_completo.pdf Versión publicada 1349758 http://zaguan.unizar.es/record/162105/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:162105 articulos driver 2025-10-09-13:25:56 ARTICLE