000162133 001__ 162133
000162133 005__ 20251017144635.0
000162133 0247_ $$2doi$$a10.3389/fmicb.2023.1232490
000162133 0248_ $$2sideral$$a134955
000162133 037__ $$aART-2023-134955
000162133 041__ $$aeng
000162133 100__ $$0(orcid)0000-0002-3017-3012$$aBernad-Roche, María
000162133 245__ $$aBuilding a predictive model for assessing the risk of Salmonella shedding at slaughter in fattening pigs
000162133 260__ $$c2023
000162133 5060_ $$aAccess copy available to the general public$$fUnrestricted
000162133 5203_ $$aSalmonellosis continues to be a major cause of foodborne outbreaks worldwide, and pigs are one of the main sources of human infection. Salmonella pork contamination is a major concern for abattoirs and is related to the presence of Salmonella in pigs' feces at slaughter. Being able to predict the risk of Salmonella shedding in pigs arriving at the slaughterhouse could help mitigate abattoir and carcass contamination. For this purpose, 30 batches of 50 pigs each were selected from 30 different fattening units. The pigs were tagged and bled for the detection of antibodies against Salmonella approximately one month before slaughter. Pooled floor fecal samples were also collected from 10 pens per unit for Salmonella detection, and a questionnaire on biosecurity was administered to each farm. At the abattoir, colon content was collected from each tagged pig for the Salmonella shedding assessment. A predictive model for Salmonella shedding at slaughter was built with two-third of the pigs by employing random-effects logistic regression analysis, with Salmonella shedding as the dependent variable and pig serology and other farm/environmental characteristics as the independent variables. The model included farm as the grouping factor. Data from the remaining one-third of the pigs were used for model validation. Out of 1,500 pigs initially selected, 1,341 were identified at the abattoir and analyzed. Salmonella was detected in 13 (43.3%; 95%CI = 27.4–60.8) of the fattening units. The mean batch seroprevalence (cut-off OD% ≥40) among the fattening units was 31.7% (95%CI = 21.8–41.0), and a total of 316 pigs (23.6%; 95%CI = 21.4–25.9) shed Salmonella at slaughter. The model predicted reasonably well (Area under the curve = 0.76; P < 0.05) whether a pig would shed Salmonella at slaughter, with estimates of sensitivity and specificity at 71.6% and 73.6%, respectively. Serology, the percentage of Salmonella-positive pens on the farm, and the internal biosecurity score were significantly associated (P < 0.05) with Salmonella shedding at the abattoir, and several scenarios were observed by the model. The study highlighted that although serology may be helpful for identifying batches of pigs at risk of shedding Salmonella upon their arrival at the abattoir, it may not be necessary in some scenarios.
000162133 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-093915-B-I00
000162133 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000162133 590__ $$a4.0$$b2023
000162133 591__ $$aMICROBIOLOGY$$b53 / 161 = 0.329$$c2023$$dQ2$$eT1
000162133 592__ $$a1.065$$b2023
000162133 593__ $$aMicrobiology (medical)$$c2023$$dQ1
000162133 593__ $$aMicrobiology$$c2023$$dQ1
000162133 594__ $$a7.7$$b2023
000162133 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000162133 700__ $$0(orcid)0000-0002-1974-9025$$aMarín-Alcalá, Clara María
000162133 700__ $$0(orcid)0000-0002-3312-7759$$aCebollada-Solanas, Alberto
000162133 700__ $$0(orcid)0000-0002-1204-4356$$ade Blas, Ignacio$$uUniversidad de Zaragoza
000162133 700__ $$0(orcid)0000-0001-5442-7702$$aMainar-Jaime, Raúl Carlos$$uUniversidad de Zaragoza
000162133 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000162133 773__ $$g14 (2023), 1232490 [11 pp.]$$pFront. microbiol.$$tFrontiers in Microbiology$$x1664-302X
000162133 8564_ $$s992844$$uhttps://zaguan.unizar.es/record/162133/files/texto_completo.pdf$$yVersión publicada
000162133 8564_ $$s2247716$$uhttps://zaguan.unizar.es/record/162133/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000162133 909CO $$ooai:zaguan.unizar.es:162133$$particulos$$pdriver
000162133 951__ $$a2025-10-17-14:28:54
000162133 980__ $$aARTICLE