000162184 001__ 162184
000162184 005__ 20251017144558.0
000162184 0247_ $$2doi$$a10.1186/s13567-025-01566-0
000162184 0248_ $$2sideral$$a144797
000162184 037__ $$aART-2025-144797
000162184 041__ $$aeng
000162184 100__ $$0(orcid)0000-0002-2079-0711$$aPérez-Lázaro, Sonia
000162184 245__ $$aDysregulated microRNAs in blood correlate with central nervous system neuropathology of prion disease
000162184 260__ $$c2025
000162184 5060_ $$aAccess copy available to the general public$$fUnrestricted
000162184 5203_ $$aThe role of microRNAs (miRNAs) in neurodegenerative diseases has gained significant attention because of their involvement in gene regulation and potential as biomarkers. In prion diseases, including scrapie, miRNAs may modulate pathogenesis and disease progression. This study investigated circulating miRNA profiles in the blood of sheep naturally affected by scrapie at preclinical and clinical stages using small RNA sequencing and RT-qPCR validation. While only one novel miRNA was dysregulated in preclinical blood samples, 66 previously annotated miRNAs were significantly dysregulated in clinical sheep compared with healthy sheep. These miRNAs are associated with pathways commonly altered in neurodegenerative diseases, such as autophagy, ubiquitin-mediated proteolysis, and endoplasmic reticulum protein processing. Notably, miR-1271-5p, let-7f-5p, miR-186-5p, and miR-425-5p were consistently upregulated in the central nervous system of clinical animals, replicating the results observed in blood, with an increasing trend already in the preclinical stage and a strong correlation with neuropathological prion features. Additionally, predicted target genes such as UBQLN2, PGK1, KRAS, and CLTC were inversely expressed relative to these miRNAs, supporting their regulatory roles. These findings highlight the relevance of circulating miRNAs in prion neuropathology and support further research into the specific functional roles of these miRNAs and their predictive capacity for disease progression.
000162184 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-125398OB-I00
000162184 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000162184 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000162184 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, Inmaculada$$uUniversidad de Zaragoza
000162184 700__ $$aCozzuto, Luca
000162184 700__ $$aPonomarenko, Julia
000162184 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan J.$$uUniversidad de Zaragoza
000162184 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, Rosa$$uUniversidad de Zaragoza
000162184 700__ $$0(orcid)0000-0002-7243-1737$$aToivonen, Janne M.$$uUniversidad de Zaragoza
000162184 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000162184 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000162184 773__ $$g56, 1 (2025), 18 pp.$$pVet. res.$$tVeterinary Research$$x0928-4249
000162184 8564_ $$s2772056$$uhttps://zaguan.unizar.es/record/162184/files/texto_completo.pdf$$yVersión publicada
000162184 8564_ $$s2123252$$uhttps://zaguan.unizar.es/record/162184/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000162184 909CO $$ooai:zaguan.unizar.es:162184$$particulos$$pdriver
000162184 951__ $$a2025-10-17-14:13:41
000162184 980__ $$aARTICLE