000162205 001__ 162205
000162205 005__ 20251017144607.0
000162205 0247_ $$2doi$$a10.1016/j.vprsr.2021.100541
000162205 0248_ $$2sideral$$a123298
000162205 037__ $$aART-2021-123298
000162205 041__ $$aeng
000162205 100__ $$0(orcid)0000-0001-6209-4282$$aVillanueva-Saz, S.$$uUniversidad de Zaragoza
000162205 245__ $$aPrevalence of microfilariae, antigen and antibodies of feline dirofilariosis infection (Dirofilaria immitis) in the Zaragoza metropolitan area, Spain
000162205 260__ $$c2021
000162205 5060_ $$aAccess copy available to the general public$$fUnrestricted
000162205 5203_ $$aFeline heartworm disease is a vector-borne parasitical disease caused by Dirofilaria immitis. Heartworm infection in dogs is prevalent in the Mediterranean countries. Information about the geographical distribution and epidemiological features of D. immitis infection in cats is scarce, particularly in urban stray cats that live within endemic regions for canine heartworm disease. The aim of the current study was to determine the seroprevalence of antigen and antibodies to D. immitis in feral cats in Zaragoza city, an endemic region of Spain. For this purpose, blood samples were examined for microfilariae using a direct blood smear technique and the modified Knott test. Two serological techniques for anti-D. immitis antibody detection (Solo Step® FH and in-house ELISA) and three different commercial antigen tests (DiroChek®, MegaELISA® DIRO Antigen and FASTest® HW) were performed. Blood samples from 250 stray cats were tested: 61 cats (24.40%) tested positive by the in-house ELISA, and 9 cats gave positive (3.6%) results with Solo Step® FH. The global seroprevalence of D. immitis in the feline population of the studied area of Zaragoza was 25.20% (63/250) including Solo Step® FH result and in-house ELISA. The blood exam for all samples was negative when evaluating for microfilariae and not a single cat was positive for antigen testing. This study demonstrates the presence of D. immitis infection in Zaragoza city. Veterinarians working in endemic areas should be aware of this infection in cats at risk and their susceptibility.
000162205 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000162205 592__ $$a0.52$$b2021
000162205 593__ $$aVeterinary (miscellaneous)$$c2021$$dQ1
000162205 593__ $$aParasitology$$c2021$$dQ1
000162205 594__ $$a2.6$$b2021
000162205 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000162205 700__ $$aGiner, J.
000162205 700__ $$0(orcid)0000-0003-2957-1379$$aVerde, M.$$uUniversidad de Zaragoza
000162205 700__ $$aYzuel, A.
000162205 700__ $$0(orcid)0000-0002-8727-0323$$aGonzález, A.
000162205 700__ $$0(orcid)0000-0002-7822-6646$$aLacasta, D.$$uUniversidad de Zaragoza
000162205 700__ $$aMarteles, D.
000162205 700__ $$0(orcid)0000-0002-2557-4890$$aFernández, A.$$uUniversidad de Zaragoza
000162205 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000162205 773__ $$g23 (2021), 100541 [5 pp.]$$tVeterinary Parasitology: Regional Studies and Reports$$x2405-9390
000162205 8564_ $$s411667$$uhttps://zaguan.unizar.es/record/162205/files/texto_completo.pdf$$yVersión publicada
000162205 8564_ $$s2799720$$uhttps://zaguan.unizar.es/record/162205/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000162205 909CO $$ooai:zaguan.unizar.es:162205$$particulos$$pdriver
000162205 951__ $$a2025-10-17-14:15:52
000162205 980__ $$aARTICLE