162206 20251017144608.0 doi 10.1111/tbed.14062 sideral 124394 ART-2022-124394 eng Villanueva-Saz, S. Universidad de Zaragoza (orcid)0000-0001-6209-4282 Serological evidence of SARS-CoV-2 and co-infections in stray cats in Spain 2022 Access copy available to the general public Unrestricted A new coronavirus known as SARS-CoV-2 emerged in Wuhan in 2019 and spread rapidly to the rest of the world causing the pandemic disease named coronavirus disease of 2019 (COVID-19). Little information is known about the impact this virus can cause upon domestic and stray animals. The potential impact of SARS-CoV-2 has become of great interest in cats due to transmission among domestic cats and the severe phenotypes described recently in a domestic cat. In this context, there is a public health warning that needs to be investigated in relation with the epidemiological role of this virus in stray cats. Consequently, in order to know the impact of the possible transmission chain, blood samples were obtained from 114 stray cats in the city of Zaragoza (Spain) and tested for SARS-CoV-2 and other selected pathogens susceptible to immunosuppression including Toxoplasma gondii, Leishmania infantum, feline leukaemia virus (FeLV) and feline immunodeficiency virus (FIV) from January to October 2020. Four cats (3.51%), based on enzyme-linked immunosorbent assay (ELISA) using the receptor binding domain (RBD) of Spike antigen, were seroreactive to SARS-CoV-2. T. gondii, L. infantum, FeLV and FIV seroprevalence was 12.28%, 16.67%, 4.39% and 19.30%, respectively. Among seropositive cats to SARS-CoV-2, three cats were also seropositive to other pathogens including antibodies detected against T. gondii and FIV (n = 1); T. gondii (n = 1); and FIV and L. infantum (n = 1). The subjects giving positive for SARS-CoV-2 were captured in urban areas of the city in different months: January 2020 (2/4), February 2020 (1/4) and July 2020 (1/4). This study revealed, for the first time, the exposure of stray cats to SARS-CoV-2 in Spain and the existence of concomitant infections with other pathogens including T. gondii, L. infantum and FIV, suggesting that immunosuppressed animals might be especially susceptible to SARS-CoV-2 infection. info:eu-repo/grantAgreement/ES/DGA-FEDER/B29-17R info:eu-repo/grantAgreement/ES/DGA-FEDER/E34-R17 info:eu-repo/grantAgreement/ES/DGA/LMP58-18 info:eu-repo/grantAgreement/ES/ISCIII/COV20-0030 info:eu-repo/grantAgreement/ES/MICINN/BFU2016-75633-P info:eu-repo/grantAgreement/ES/MICINN/CTQ2013-44367-C2-2-P info:eu-repo/grantAgreement/ES/MICINN/PID2019-105451GB-I00 info:eu-repo/grantAgreement/ES/MICINN/SAF2017-83120-C2-1-R info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 4.3 2022 VETERINARY SCIENCES 7 / 144 = 0.049 2022 Q1 T1 INFECTIOUS DISEASES 42 / 96 = 0.438 2022 Q2 T2 0.99 2022 Medicine (miscellaneous) 2022 Q1 Veterinary (miscellaneous) 2022 Q1 Immunology and Microbiology (miscellaneous) 2022 Q2 7.4 2022 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Giner, J. Tobajas, A.P. Universidad de Zaragoza (orcid)0000-0002-2646-5733 Pérez, M.D. Universidad de Zaragoza (orcid)0000-0003-2555-8425 González-Ramírez, A.M. Universidad de Zaragoza (orcid)0000-0002-5838-0857 Macías-León, J. Universidad de Zaragoza (orcid)0000-0001-6815-6720 González, A. Verde, M. Universidad de Zaragoza (orcid)0000-0003-2957-1379 Yzuel, A. Hurtado-Guerrero, R. (orcid)0000-0002-3122-9401 Pardo, J. Universidad de Zaragoza (orcid)0000-0003-0154-0730 Santiago, L. (orcid)0000-0002-1861-5981 Paño-Pardo, J.R. Universidad de Zaragoza (orcid)0000-0002-9600-8116 Ruíz, H. Universidad de Zaragoza (orcid)0000-0002-8474-2831 Lacasta, D.M. Universidad de Zaragoza (orcid)0000-0002-7822-6646 Sánchez, L. Universidad de Zaragoza (orcid)0000-0001-5964-823X Marteles, D. Gracia, A.P. (orcid)0000-0001-9333-3055 Fernández, A. Universidad de Zaragoza (orcid)0000-0002-2557-4890 1011 566 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Inmunología 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 69 (2022), 1056-1064 Transboundary and Emerging Diseases Transboundary and Emerging Diseases 1865-1674 582071 http://zaguan.unizar.es/record/162206/files/texto_completo.pdf Postprint 951409 http://zaguan.unizar.es/record/162206/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:162206 articulos driver 2025-10-17-14:16:03 ARTICLE