000162235 001__ 162235
000162235 005__ 20251017144634.0
000162235 0247_ $$2doi$$a10.1007/s11259-022-10016-7
000162235 0248_ $$2sideral$$a131228
000162235 037__ $$aART-2023-131228
000162235 041__ $$aeng
000162235 100__ $$0(orcid)0000-0001-6209-4282$$aVillanueva-Saz, Sergio$$uUniversidad de Zaragoza
000162235 245__ $$aA cross-sectional serosurvey of SARS-CoV-2 and co-infections in stray cats from the second wave to the sixth wave of COVID-19 outbreaks in Spain
000162235 260__ $$c2023
000162235 5060_ $$aAccess copy available to the general public$$fUnrestricted
000162235 5203_ $$aSevere Acute Respiratory Syndrome Coronavirus 2 is the causative agent of Coronavirus Disease 2019 in humans. Among domestic animals, cats are more susceptible to SARS-CoV-2 than dogs. The detection of anti-SARS-CoV-2 antibodies in seemingly healthy cats and/or infected cats which are in close contact with infected humans has been described. The presence of animals that tested positive by serology or molecular techniques could represent a potential transmission pathway of SARS-CoV-2 that can spill over into urban wildlife. This study analyses the seroprevalence variation of SARS-CoV-2 in stray cats from different waves of outbreaks in a geographical area where previous seroepidemiological information of SARS-CoV-2 was available and investigate if SARS-CoV-2-seropositive cats were exposed to other co-infections causing an immunosuppressive status and/or a chronic disease that could lead to a SARS-CoV-2 susceptibility. For this purpose, a total of 254 stray cats from Zaragoza (Spain) were included. This analysis was carried out by the enzyme-linked immunosorbent assay using the receptor binding domain of Spike antigen and confirmed by serum virus neutralization assay. The presence of co-infections including Toxoplasma gondii, Leishmania infantum, Dirofilaria immitis, feline calicivirus, feline herpesvirus type 1, feline leukemia virus and feline immunodeficiency virus, was evaluated using different serological methods. A seropositivity of 1.57% was observed for SARS-CoV-2 including the presence of neutralizing antibodies in three cats. None of the seropositive to SARS-CoV-2 cats were positive to feline coronavirus, however, four SARS-CoV-2-seropositive cats were also seropositive to other pathogens such as L. infantum, D. immitis and FIV (n = 1), L. infantum and D. immitis (n = 1) and L. infantum alone (n = 1).Considering other pathogens, a seroprevalence of 16.54% was detected for L. infantum, 30.31% for D. immitis, 13.78%, for T. gondii, 83.86% for feline calicivirus, 42.52% for feline herpesvirus type 1, 3.15% for FeLV and 7.87% for FIV.
Our findings suggest that the epidemiological role of stray cats in SARS-CoV-2 transmission is scarce, and there is no increase in seropositivity during the different waves of COVID-19 outbreaks in this group of animals. Further epidemiological surveillances are necessary to determine the risk that other animals might possess even though stray cats do not seem to play a role in transmission.
000162235 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000162235 590__ $$a1.8$$b2023
000162235 591__ $$aVETERINARY SCIENCES$$b53 / 167 = 0.317$$c2023$$dQ2$$eT1
000162235 592__ $$a0.483$$b2023
000162235 593__ $$aVeterinary (miscellaneous)$$c2023$$dQ2
000162235 593__ $$aMedicine (miscellaneous)$$c2023$$dQ3
000162235 594__ $$a2.5$$b2023
000162235 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000162235 700__ $$0(orcid)0000-0002-9071-1919$$aMartínez, Mariví
000162235 700__ $$aGiner, Jacobo
000162235 700__ $$0(orcid)0000-0002-8727-0323$$aGonzález, Ana
000162235 700__ $$0(orcid)0000-0002-2646-5733$$aTobajas, Ana Pilar$$uUniversidad de Zaragoza
000162235 700__ $$0(orcid)0000-0003-2555-8425$$aPérez, María Dolores$$uUniversidad de Zaragoza
000162235 700__ $$aLira-Navarrete, Erandi
000162235 700__ $$0(orcid)0000-0002-5838-0857$$aGonzález-Ramírez, Andrés Manuel$$uUniversidad de Zaragoza
000162235 700__ $$0(orcid)0000-0001-6815-6720$$aMacías-León, Javier
000162235 700__ $$0(orcid)0000-0003-2957-1379$$aVerde, Maite$$uUniversidad de Zaragoza
000162235 700__ $$aYzuel, Andrés
000162235 700__ $$0(orcid)0000-0002-3122-9401$$aHurtado-Guerrero, Ramón
000162235 700__ $$0(orcid)0000-0002-9730-2210$$aArias, Maykel
000162235 700__ $$0(orcid)0000-0002-1861-5981$$aSantiago, Llipsy
000162235 700__ $$aAguiló-Gisbert, Jordi
000162235 700__ $$0(orcid)0000-0002-8474-2831$$aRuíz, Héctor$$uUniversidad de Zaragoza
000162235 700__ $$0(orcid)0000-0002-7822-6646$$aLacasta, Delia$$uUniversidad de Zaragoza
000162235 700__ $$aMarteles, Diana$$uUniversidad de Zaragoza
000162235 700__ $$0(orcid)0000-0002-2557-4890$$aFernández, Antonio$$uUniversidad de Zaragoza
000162235 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000162235 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000162235 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000162235 773__ $$g47, 2 (2023), 615–629$$pVet. res. commun.$$tVeterinary Research Communications$$x0165-7380
000162235 8564_ $$s383733$$uhttps://zaguan.unizar.es/record/162235/files/texto_completo.pdf$$yPostprint
000162235 8564_ $$s1255893$$uhttps://zaguan.unizar.es/record/162235/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000162235 909CO $$ooai:zaguan.unizar.es:162235$$particulos$$pdriver
000162235 951__ $$a2025-10-17-14:28:16
000162235 980__ $$aARTICLE