000162237 001__ 162237
000162237 005__ 20251017144636.0
000162237 0247_ $$2doi$$a10.1111/vcp.13230
000162237 0248_ $$2sideral$$a133338
000162237 037__ $$aART-2023-133338
000162237 041__ $$aeng
000162237 100__ $$0(orcid)0000-0001-6209-4282$$aVillanueva-Saz, Sergio$$uUniversidad de Zaragoza
000162237 245__ $$aEvaluation of an immunochromatographic serologic test to detect the presence of anti-Toxoplasma gondii antibodies in cats
000162237 260__ $$c2023
000162237 5060_ $$aAccess copy available to the general public$$fUnrestricted
000162237 5203_ $$aBackground: Toxoplasmosis is a protozoan disease caused by Toxoplasma gondii. Different T. gondii confirmatory techniques, including serologic methods, are available to detect the presence of the parasite. Among serology techniques, immunochromatographic rapid testing could be a reliable alternative to serologic laboratory techniques.
Objective: This study evaluated a commercial immunochromatographic test (FASTest TOXOPLASMA g) in seronegative and seropositive cats.
Methods: Two indirect immunofluorescence antibody reference tests, an in-house technique, and a commercial test were used to classify 292 feline serum samples. The rapid test was evaluated in different groups of cats, including healthy seronegative cats (n = 121), seropositive cats with variable anti-Toxoplasma antibodies (n = 146), and cats with positive serologic results for other pathogens (n = 25). The sensitivity, specificity, accuracy, receiver operating characteristic curves, and kappa statistics were analyzed as performance measures.
Results: Of the 292 samples, 146 were classified as T. gondii seropositive and 146 as T. gondii seronegative. Concordant results were obtained for all samples using immunofluorescence antibody tests. The diagnostic measures of this rapid test showed 98.63% sensitivity and 100% specificity, and 99.32% accuracy. The kappa statistics value was 0.986, and the area under the receiver operating characteristic curve was 0.993.
Conclusions: This rapid test showed diagnostic measurements similar to those of traditional quantitative serologic methods. In situations where laboratory techniques are not available, this test, under clinical conditions, could be a useful alternative to obtain accurate results rapidly.
000162237 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttps://creativecommons.org/licenses/by-nc/4.0/deed.es
000162237 590__ $$a1.2$$b2023
000162237 591__ $$aVETERINARY SCIENCES$$b90 / 167 = 0.539$$c2023$$dQ3$$eT2
000162237 592__ $$a0.421$$b2023
000162237 593__ $$aVeterinary (miscellaneous)$$c2023$$dQ2
000162237 593__ $$aMedicine (miscellaneous)$$c2023$$dQ3
000162237 594__ $$a1.7$$b2023
000162237 655_4 $$ainfo:eu-repo/semantics/conferenceObject$$vinfo:eu-repo/semantics/publishedVersion
000162237 700__ $$aMartínez, Mariví
000162237 700__ $$aGiner, Jacobo
000162237 700__ $$0(orcid)0000-0003-2555-8425$$aPérez, María Dolores$$uUniversidad de Zaragoza
000162237 700__ $$0(orcid)0000-0002-2646-5733$$aTobajas, Ana Pilar$$uUniversidad de Zaragoza
000162237 700__ $$aYzuel, Andrés
000162237 700__ $$0(orcid)0000-0003-2957-1379$$aVerde, María Teresa$$uUniversidad de Zaragoza
000162237 700__ $$0(orcid)0000-0002-7822-6646$$aLacasta, Delia$$uUniversidad de Zaragoza
000162237 700__ $$0(orcid)0000-0002-2557-4890$$aFernández, Antonio$$uUniversidad de Zaragoza
000162237 700__ $$aMarteles, Diana$$uUniversidad de Zaragoza
000162237 700__ $$0(orcid)0000-0002-8474-2831$$aRuíz, Héctor$$uUniversidad de Zaragoza
000162237 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000162237 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000162237 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000162237 773__ $$g52, 2 (2023), 284-287$$pVet. clin. pathol.$$tVETERINARY CLINICAL PATHOLOGY$$x0275-6382
000162237 8564_ $$s462874$$uhttps://zaguan.unizar.es/record/162237/files/texto_completo.pdf$$yVersión publicada
000162237 8564_ $$s2051607$$uhttps://zaguan.unizar.es/record/162237/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000162237 909CO $$ooai:zaguan.unizar.es:162237$$particulos$$pdriver
000162237 951__ $$a2025-10-17-14:29:06
000162237 980__ $$aARTICLE