162244 20251017144644.0 doi 10.1016/j.prenap.2025.100304 sideral 144793 ART-2025-144793 eng Botelho, Cláudia Characterizing the phenolic composition and exploring the antioxidant, anti-cancer, and anti-inflammatory potential of medicinal plant infusions 2025 Access copy available to the general public Unrestricted Natural products and their derivatives have played important roles in various fields, including nutrition, medicine and cosmetics. In this study, different formulated plant infusions, namely Purifying Body (PB), Boost Training (BT), Calm Night I Spirit (CN), and Boost Energy (BE) were analyzed regarding their phenolic composition and biological properties such as antioxidant, anti-inflammatory, and its influence on a different type of tumoral and non-tumoral cells. Nineteen phenolic compounds across six groups were identified, being cinnamic acid the most prevalent in all infusions. BE infusions exhibited the highest total phenolic content (5 g GAE/L), while BT had the lowest (2 g GAE/L). BE demonstrated superior antioxidant activity, correlating with its high phenolic content. The impact of plant infusions on cell metabolic activity revealing a concentration-dependent behaviour for three used cell lines [human embryonic kidney (HEK293t), normal mouse fibroblast (L929), and human colorectal adenocarcinoma (Caco-2)]. IC50 values indicated low toxicity on non-tumoral cells and higher inhibition on the metabolic activity of colon cancer Caco-2 cells. These effects can be due to the induction of cell cycle arrest and apoptosis. All plant infusions significantly prevented reactive oxygen species formation (oxidative stress regulation), and present anti-inflammatory capacity with a decrease of pro-inflammatory (IL-1β) and an increase of anti-inflammatory (IL-10) cytokines. Overall, the formulated infusions have antioxidant and anti-inflammatory properties and a notable impact on colon cancer cells. This study revealed the potential of the tested infusions in various medical applications, such as preventive care, as well as in the nutraceutical field. info:eu-repo/grantAgreement/ES/AEI/FJC2021-046978-I info:eu-repo/grantAgreement/ES/DGA/B16-23R info:eu-repo/grantAgreement/ES/ISCIII/CB06-03-1012 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Ferreira-Sousa, Diana Quero, Javier Costa-Barbosa, Augusto Teixeira-Guedes, Catarina Sampaio, Paula Rodríguez-Yoldi, María Jesús Universidad de Zaragoza (orcid)0000-0002-3595-7668 Ferreira-Santos, Pedro 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 8 (2025), 100304 [13 pp.] PRENAP Pharmacological Research. Natural Products 2950-1997 1059103 http://zaguan.unizar.es/record/162244/files/texto_completo.pdf Postprint 1117517 http://zaguan.unizar.es/record/162244/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:162244 articulos driver 2025-10-17-14:33:24 ARTICLE