000162256 001__ 162256 000162256 005__ 20251017144551.0 000162256 0247_ $$2doi$$a10.1016/j.intimp.2019.105674 000162256 0248_ $$2sideral$$a114391 000162256 037__ $$aART-2019-114391 000162256 041__ $$aeng 000162256 100__ $$aMarteles, Diana$$uUniversidad de Zaragoza 000162256 245__ $$aEffects of allergen-specific immunotherapy on peripheral blood regulatory T cells and serum concentrations of cytokines and immunoglobulins in horses with allergic dermatitis 000162256 260__ $$c2019 000162256 5060_ $$aAccess copy available to the general public$$fUnrestricted 000162256 5203_ $$aThe aim of this study was to assess the effect of allergen-specific immunotherapy (ASIT) on the immunological responses of horses. Blood samples were taken from thirty-two horses with allergic dermatitis treated with ASIT and 10 healthy control horses at 0, 3, 6, 9 and 12¿months to investigate the evolution of the percentage of regulatory T cells (Treg) in the peripheral blood and the serum levels of cytokines and immunoglobulins. Clinical improvement was appreciated by the majority of the horses' owners (56.6%). No effect of ASIT on CD4+CD25High Treg cells was found during the one year treatment period. No differences in the percentage of CD4+ T cells were observed between the groups, and no effects of ASIT over time were observed. The percentage of CD25+ T cells was always higher in the ASIT group (17.9¿±¿11.3%) than in the control group (7.3¿±¿4.4%, p¿<¿0.001). We did not detect any effect of ASIT on the serum levels of TGF-ß, IL-10 and IFN-¿ or on the serum concentrations of IgA and IgG4. A reduction in the serum levels of total IgE in the horses with allergic dermatitis was observed at the 6th month (p¿<¿0.05), but increased again at the end of the study. The results indicate that immunotherapy was insufficient to induce significant changes that could indicate T cell tolerance, a shift in cytokine production to more protective Th1 cells. More studies are needed with new vaccine compositions and administration protocols to improve the immunological responses of the horses with allergic dermatitis. 000162256 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 000162256 590__ $$a3.943$$b2019 000162256 591__ $$aPHARMACOLOGY & PHARMACY$$b63 / 270 = 0.233$$c2019$$dQ1$$eT1 000162256 591__ $$aIMMUNOLOGY$$b60 / 158 = 0.38$$c2019$$dQ2$$eT2 000162256 592__ $$a0.992$$b2019 000162256 593__ $$aPharmacology$$c2019$$dQ1 000162256 593__ $$aImmunology$$c2019$$dQ2 000162256 593__ $$aImmunology and Allergy$$c2019$$dQ2 000162256 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000162256 700__ $$0(orcid)0000-0003-2957-1379$$aVerde, María Teresa$$uUniversidad de Zaragoza 000162256 700__ $$0(orcid)0000-0002-9500-4703$$aConde, Tomás$$uUniversidad de Zaragoza 000162256 700__ $$0(orcid)0000-0002-9357-143X$$aPereboom Maicas, Matilde Desirée$$uUniversidad de Zaragoza 000162256 700__ $$0(orcid)0000-0002-1273-754X$$aCasanova, Álvaro$$uUniversidad de Zaragoza 000162256 700__ $$0(orcid)0000-0001-6209-4282$$aVillanueva-Saz, Sergio$$uUniversidad de Zaragoza 000162256 700__ $$0(orcid)0000-0002-0313-5926$$aOrtín, Aurora$$uUniversidad de Zaragoza 000162256 700__ $$0(orcid)0000-0002-2557-4890$$aFernández, Antonio$$uUniversidad de Zaragoza 000162256 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología 000162256 7102_ $$11005$$2315$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Farmacología 000162256 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal 000162256 773__ $$g74 (2019), 105674 [8 pp.]$$pInt. Immunopharmacol.$$tINTERNATIONAL IMMUNOPHARMACOLOGY$$x1567-5769 000162256 8564_ $$s659220$$uhttps://zaguan.unizar.es/record/162256/files/texto_completo.pdf$$yPostprint 000162256 8564_ $$s773767$$uhttps://zaguan.unizar.es/record/162256/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000162256 909CO $$ooai:zaguan.unizar.es:162256$$particulos$$pdriver 000162256 951__ $$a2025-10-17-14:11:53 000162256 980__ $$aARTICLE