162256 20251017144551.0 doi 10.1016/j.intimp.2019.105674 sideral 114391 ART-2019-114391 eng Marteles, Diana Universidad de Zaragoza Effects of allergen-specific immunotherapy on peripheral blood regulatory T cells and serum concentrations of cytokines and immunoglobulins in horses with allergic dermatitis 2019 Access copy available to the general public Unrestricted The aim of this study was to assess the effect of allergen-specific immunotherapy (ASIT) on the immunological responses of horses. Blood samples were taken from thirty-two horses with allergic dermatitis treated with ASIT and 10 healthy control horses at 0, 3, 6, 9 and 12¿months to investigate the evolution of the percentage of regulatory T cells (Treg) in the peripheral blood and the serum levels of cytokines and immunoglobulins. Clinical improvement was appreciated by the majority of the horses' owners (56.6%). No effect of ASIT on CD4+CD25High Treg cells was found during the one year treatment period. No differences in the percentage of CD4+ T cells were observed between the groups, and no effects of ASIT over time were observed. The percentage of CD25+ T cells was always higher in the ASIT group (17.9¿±¿11.3%) than in the control group (7.3¿±¿4.4%, p¿<¿0.001). We did not detect any effect of ASIT on the serum levels of TGF-ß, IL-10 and IFN-¿ or on the serum concentrations of IgA and IgG4. A reduction in the serum levels of total IgE in the horses with allergic dermatitis was observed at the 6th month (p¿<¿0.05), but increased again at the end of the study. The results indicate that immunotherapy was insufficient to induce significant changes that could indicate T cell tolerance, a shift in cytokine production to more protective Th1 cells. More studies are needed with new vaccine compositions and administration protocols to improve the immunological responses of the horses with allergic dermatitis. info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 3.943 2019 PHARMACOLOGY & PHARMACY 63 / 270 = 0.233 2019 Q1 T1 IMMUNOLOGY 60 / 158 = 0.38 2019 Q2 T2 0.992 2019 Pharmacology 2019 Q1 Immunology 2019 Q2 Immunology and Allergy 2019 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Verde, María Teresa Universidad de Zaragoza (orcid)0000-0003-2957-1379 Conde, Tomás Universidad de Zaragoza (orcid)0000-0002-9500-4703 Pereboom Maicas, Matilde Desirée Universidad de Zaragoza (orcid)0000-0002-9357-143X Casanova, Álvaro Universidad de Zaragoza (orcid)0000-0002-1273-754X Villanueva-Saz, Sergio Universidad de Zaragoza (orcid)0000-0001-6209-4282 Ortín, Aurora Universidad de Zaragoza (orcid)0000-0002-0313-5926 Fernández, Antonio Universidad de Zaragoza (orcid)0000-0002-2557-4890 1005 410 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Fisiología 1005 315 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Farmacología 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 74 (2019), 105674 [8 pp.] Int. Immunopharmacol. INTERNATIONAL IMMUNOPHARMACOLOGY 1567-5769 659220 http://zaguan.unizar.es/record/162256/files/texto_completo.pdf Postprint 773767 http://zaguan.unizar.es/record/162256/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:162256 articulos driver 2025-10-17-14:11:53 ARTICLE