162298 20251017144606.0 doi 10.1038/s41598-025-09822-1 sideral 144870 ART-2025-144870 eng Paesa, Mónica Universidad de Zaragoza Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatment 2025 Access copy available to the general public Unrestricted Osteoarthritis (OA) is a joint disease characterized by inflammation, cartilage degeneration, and pain. Recent studies have focused on the potential of active phenolic compounds as a treatment for OA, owing to their anti-inflammatory, antioxidant, and chondroprotective effects. Specifically, Boletus edulis (BE) extracts, a natural product from mushrooms, have been shown as significantly active compounds against oxidative stress and inflammation. Our aim was to evaluate the anti-inflammatory and chondroprotective potential of BE extracts, primarily composed of phenolic compounds and flavonoids, in OA in vitro models. Our results showed that cell treatment with 250 µg/mL of BE extracts maintained cell viability above 70% without affecting apoptosis or cell cycle. At the molecular level, BE extracts exerted anti-inflammatory effects by reducing nitric oxide (NO) levels, as well as the expression of inducible nitric oxide synthase (iNOS) and pro-inflammatory cytokines and chemokines (interleukin-6 (IL-6), IL-8, (C-X-C motif) ligand 1 (CXCL-1)) in lipopolysaccharide (LPS)-stimulated cells. Additionally, BE extracts treatment demonstrated a significant decrease in matrix metalloproteinases 3 and 13 (MMP-3, MMP-13) expression, while avoiding the depletion in the expression of chondrogenic markers, aggrecan and collagen II. Furthermore, inflammation was also significantly reduced through the inhibition of p65 nuclear translocation. These findings suggest that BE extracts may provide a promising basis for the development of novel therapeutic strategies in the treatment of OA, reducing inflammation and chondrocytes damage. info:eu-repo/grantAgreement/ES/DGA/B55 info:eu-repo/grantAgreement/ES/IISAragón/FSE-I-01/Intramural23 info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI21-00911 info:eu-repo/grantAgreement/ES/ISCIII/FORT23-00028 info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Izquierdo, Mauricio David Remírez de Ganuza, Cristina Universidad de Zaragoza Ferreira-Santos, Pedro Rodríguez-Yoldi, María Jesús Universidad de Zaragoza (orcid)0000-0002-3595-7668 Mendoza, Gracia (orcid)0000-0003-2293-363X 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 5005 790 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Tecnologi. Medio Ambiente 15, 1 (2025), 15 pp. Sci. rep. (Nat. Publ. Group) Scientific reports (Nature Publishing Group) 2045-2322 2435811 http://zaguan.unizar.es/record/162298/files/texto_completo.pdf Versión publicada 2525887 http://zaguan.unizar.es/record/162298/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:162298 articulos driver 2025-10-17-14:15:41 ARTICLE