162299 20251017144606.0 doi 10.1038/s41541-025-01201-1 sideral 144878 ART-2025-144878 eng Mata, Elena Preserved efficacy of lyophilized SARS-CoV-2 mRNA vaccine incorporating novel ionizable lipids after one year at 25 °C 2025 Access copy available to the general public Unrestricted mRNA vaccines have shown great efficacy against SARS-CoV-2, yet challenges remain in optimizing vaccine components to achieve enhanced immune response and vaccine stability. In this study, we developed CPVax-CoV, a new lyophilized mRNA vaccine that features novel thiolactone-based ionizable lipids and newly designed untranslated regions (UTRs) for enhanced expression. Incorporation of these optimized components into our vaccine candidate CPVax-CoV significantly improved immune responses in mice compared to commercially available mRNA vaccines. Moreover, lyophilized CPVax-CoV has proven to be thermostable, maintaining its biological activity for up to one year at 4 °C and 25 °C after lyophilization, overcoming the cold-chain limitations of current mRNA vaccines. This vaccine demonstrates protective efficacy against ancestral SARS-CoV-2 and the Omicron XBB variant, offering a scalable solution for global distribution and pandemic preparedness. These findings underscore the potential of this platform for future next-generation mRNA vaccine development. info:eu-repo/grantAgreement/ES/AEI/PID2020-113963RB-I00 info:eu-repo/grantAgreement/ES/DGA/B29-20R info:eu-repo/grantAgreement/ES/ISCIII/CB21-13-00087 info:eu-repo/grantAgreement/ES/ISCIII/COV20-00308 info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Broset, Esther Matute, Carlos Stoian, Andrei Adame, Susana Alejo, Teresa López, Alexandre Andrés, Beatriz Heredero, Juan de Miguel, Diego Giménez-Warren, Javier Lampaya, Verónica Casabona, Diego Calvo, Alba Quincoces, Gema Peñuelas, Iván Gamazo, Carlos Uranga, Iratxe Peña, Natacha Arias, Maykel (orcid)0000-0002-9730-2210 Pardo, Julián Universidad de Zaragoza (orcid)0000-0003-0154-0730 Moreno, Bernardino Universidad de Zaragoza (orcid)0000-0001-6629-6842 Badiola, Juan Universidad de Zaragoza (orcid)0000-0002-7173-7216 Martínez-Oliván, Juan (orcid)0000-0002-8784-7735 Pérez-Herrán, Esther 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1011 566 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Inmunología 10, 135 (2025), 19 npj Vaccines npj Vaccines 2059-0105 2928116 http://zaguan.unizar.es/record/162299/files/texto_completo.pdf Versión publicada 2814186 http://zaguan.unizar.es/record/162299/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:162299 articulos driver 2025-10-17-14:15:41 ARTICLE