162307 20251017144607.0 doi 10.36922/IJB025120094 sideral 144915 ART-2025-144915 eng Martín Compaired, Pablo Universidad de Zaragoza An experimental workflow for bioprinting optimization: Application to a custom-made biomaterial ink 2025 Access copy available to the general public Unrestricted Bioprinting is an emerging technology with significant potential in biomedical fields, enabling the creation of highly customized, cell-laden constructs. Despite the promise, achieving high-quality, reproducible prints remains challenging due to the lack of standardized protocols, which has hindered widespread adoption of the technique. In this study, we present a systematic bioprinting protocol designed to optimize the performance of an in-house photo-curable biomaterial ink composed of gelatin methacryloyl (GelMA) and egg white protein. Printing quality was evaluated through three key assessments: extrusion, deposition, and printability. To facilitate accurate image analysis, we developed a custom 3D-printed lens support specifically designed for a USB-microscope. Additionally, we implemented a Python script to quantitatively assess bioprinting quality. Our results indicate that a pressure range of 70-80 KPa, combined with speeds between 300 and 900 mm/min, yields reliable extrusion flow, with 75 KPa and 600 mm/min emerging as optimal parameters for bioprinting 3D constructs. These findings underscore the importance of carefully tuning parameters – including pressure and speed – to achieve stable, high-resolution extrusions. Such optimization mitigates common printing issues, including tip clogging, filament dragging, and unintended merging of adjacent filaments, thereby enhancing structural accuracy. This work provides a comprehensive framework for evaluating and optimizing bioprinting parameters, offering a reproducible methodology to enhance print quality. Contributing to the ongoing efforts to standardize bioprinting processes and advance their applications in tissue engineering and regenerative medicine. info:eu-repo/grantAgreement/ES/MICINN/PID2023-146072OB-I00 info:eu-repo/grantAgreement/ES/MICINN/RYC2021-033490-I info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion García-Gareta, Elena Universidad de Zaragoza (orcid)0000-0001-7062-9099 Pérez, María Ángeles Universidad de Zaragoza (orcid)0000-0002-2901-4188 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 5001 065 Universidad de Zaragoza Dpto. Ciencia Tecnol.Mater.Fl. Área Cienc.Mater. Ingen.Metal. 11, 3 (2025), 397-415 International Journal of Bioprinting 2424-7723 4201175 http://zaguan.unizar.es/record/162307/files/texto_completo.pdf Versión publicada 2214776 http://zaguan.unizar.es/record/162307/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:162307 articulos driver 2025-10-17-14:15:49 ARTICLE