162382 20251017144654.0 doi 10.1016/j.ncrna.2025.06.003 sideral 144865 ART-2025-144865 eng Aparicio, Paula (orcid)0000-0001-5612-0253 Serum lncRNAs NEAT1, PVT1 and H19 as novel biomarkers for sarcopenia diagnosis and treatment response 2025 Access copy available to the general public Unrestricted Sarcopenia, the loss of muscle mass and function generally associated to age, leads to increased dependence and mortality in older adults. Despite its clinical significance, unclear molecular mechanisms hinder the development of universal diagnostic and therapeutic monitoring methods. Recent research suggests long non-coding RNAs (lncRNAs) as potential biomarkers for muscle damage and sarcopenia. This study investigates the role of six specific lncRNAs as biomarkers for diagnosing and monitoring sarcopenia following physical training. For this purpose, an initial cohort of participants was divided into two experiments: Trial 1, a cross-sectional study comprising 54 sarcopenic patients and 29 robust controls, both including men and women; Trial 2, a non-randomized controlled trial, where the same sarcopenic patients from Trial 1 were divided in two groups: a Control Group (CG, n = 15); and a Trained Group (TG, n = 22). RNA was extracted from serum samples for all the participants, and the expression of 6 lncRNA (PVT1, HOTAIR, MALAT1, NEAT1, GAS5, H19), selected from the literature, was quantified by RT-PCR and compared between the different groups. Statistical evaluation uncovered four lncRNAs with significantly distinct expression in Trial 1: PVT1 (LOG2FC = 1.194), GAS5 (LOG2FC = 0.8224), NEAT1 (LOG2FC = 1.497) and H19 (LOG2FC = −0.9958) and three lncRNA significantly different between TG and CG in Trial 2 (PVT1 (LOG2FC = −1.796), MALAT1 (LOG2FC = 2.834) and H19 (LOG2FC = 1.355). Among them, NEAT 1 stands aout as promissing diagnostic marker ans PVT1 and H19 may serve as both diagnosis and treatment monitoring, altough further validation in larger cohorts is needed to confirm these results. info:eu-repo/grantAgreement/ES/AEI/PID2022-140911OB-I00 info:eu-repo/grantAgreement/ES/ISCIII/CB12-03-30038 info:eu-repo/grantAgreement/ES/ISCIII/CB18-05-0037 info:eu-repo/grantAgreement/ES/ISCIII/FEDER/PI21-00372 info:eu-repo/grantAgreement/ES/MICINN-AEI/PRTR-C17.I1 info:eu-repo/grantAgreement/ES/MINECO/DEP2016-78309-R info:eu-repo/grantAgreement/ES/UZ/UZCUD2017-BIO-01 info:eu-repo/grantAgreement/ES/UZ/UZ2021-BIO-05 info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion López-Royo, Tresa (orcid)0000-0001-9802-8199 Navarrete-Villanueva, David Universidad de Zaragoza (orcid)0000-0001-6523-5511 Gómez Cabello, Alba María (orcid)0000-0001-6492-2512 González-Gross, Marcela (orcid)0000-0001-7757-3235 Ara, Ignacio (orcid)0000-0002-2854-6684 Vicente-Rodríguez, Germán Universidad de Zaragoza (orcid)0000-0002-4303-4097 Osta, Rosario Universidad de Zaragoza (orcid)0000-0001-5687-6704 Manzano, Raquel Universidad de Zaragoza (orcid)0000-0002-7477-8742 1006 255 Universidad de Zaragoza Dpto. Fisiatría y Enfermería Área Enfermería 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1006 245 Universidad de Zaragoza Dpto. Fisiatría y Enfermería Área Educación Física y Depor. 14 (2025), 166-176 Non-coding RNA Research 2468-0540 3637962 http://zaguan.unizar.es/record/162382/files/texto_completo.pdf Versión publicada 2179331 http://zaguan.unizar.es/record/162382/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:162382 articulos driver 2025-10-17-14:37:36 ARTICLE