162405 20260418110659.0 doi 10.1002/1873-3468.70117 sideral 144994 ART-2025-144994 eng Di Napoli, Giulia Mycobacterium tuberculosis sulfurtransferase SseA is activated by its neighboring gene product Rv3284 2025 Access copy available to the general public Unrestricted Tuberculosis remains a critical global health challenge, which underscores the need for new therapeutic targets. A potential drug target is the rhodanese‐like thiosulfate sulfurtransferase SseA, which plays a role in macrophage infection by Mycobacterium tuberculosis (Mtb) and its resistance to oxidative stress. In our research, we identified a protein (Rv3284), herein referred to as SufEMtb, that interacts with SseA and modulates its activity. Sequence analysis and molecular modeling revealed that SufEMtb enhances SseA enzymatic function by binding to its non‐catalytic N‐terminal domain and favoring an activating conformational change in a regulatory loop of SseA. This interaction appears crucial for effective enzyme activity and the maintenance of redox homeostasis in Mtb, making the SseA–SufEMtb complex a potential target for new therapies. info:eu-repo/grantAgreement/ES/DGA-FEDER/E35-23R info:eu-repo/grantAgreement/ES/MICINN/PID2022-136369NB-I00 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Fissore, Alex Salladini, Edoardo Raccuia, Eleonora Oliaro-Bosso, Simonetta Ruggiero, Alessia Berisio, Rita Medina, Milagros Universidad de Zaragoza (orcid)0000-0001-8743-0182 Velazquez-Campoy, Adrian Universidad de Zaragoza (orcid)0000-0001-5702-4538 Adinolfi, Salvatore Marengo, Mauro 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 599, 16 (2025), 2362-2376 FEBS lett. FEBS Letters 0014-5793 2576919 http://zaguan.unizar.es/record/162405/files/texto_completo.pdf Versión publicada 2341987 http://zaguan.unizar.es/record/162405/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:162405 articulos driver 2026-04-18-11:05:08 ARTICLE