000162409 001__ 162409
000162409 005__ 20251017144601.0
000162409 0247_ $$2doi$$a10.1016/j.foodres.2025.117165
000162409 0248_ $$2sideral$$a145009
000162409 037__ $$aART-2025-145009
000162409 041__ $$aeng
000162409 100__ $$0(orcid)0000-0002-4027-5637$$aGuillén, Silvia$$uUniversidad de Zaragoza
000162409 245__ $$aThe stressosome and SigB influence the baseline thermal resistance but not dynamic adaptation of Listeria monocytogenes: insights from kinetic modelling
000162409 260__ $$c2025
000162409 5060_ $$aAccess copy available to the general public$$fUnrestricted
000162409 5203_ $$aThis study takes a first step towards incorporating a more mechanistic basis into current microbial inactivation models. We focus on the role of SigB and the stressosome in the heat resistance of Listeria monocytogenes using a reference strain (EGD-e), as well as five mutant strains where SigB and/or the stressosome were mutated. Data was analysed using the Geeraerd model to quantify the link between SigB/stressosome and thermal resistance. The ΔsigB strain had lower thermal resistance ( = 0.21 log10 min) than EGD-e (0.27 log10 min). Mutations known to negatively affect the stressosome function also reduced the D-value (0.18 log10 min for RsbS S56A). Survivor curves had also a shoulder, which was also affected by SigB/stressosome. Exponential phase cells of the RsbR1-only strain had the shortest shoulder, while the wild type strain had the longest, which suggests that RsbR1 paralogues play a role in thermal stress response. Every strain was able to develop stress acclimation during dynamic heat treatments at low heating rates (2 °C/min) suggesting that factors other than SigB are important for thermal adaptation. Its impact was comparable to the jump from exponential to stationary phase cells, underlining the relevance of stress acclimation. This emphasizes the need for predictive models to go beyond a “baseline” resistance, accounting for the ability of cells to change their resistance as a response to environmental signals. This study marks a first step towards that goal, by defining possible ranges of variation for the D-value and shoulder length of L. monocytogenes.
000162409 536__ $$9info:eu-repo/grantAgreement/ES/MICINN-AEI/PRTR-C17.I1$$9info:eu-repo/grantAgreement/ES/MICIU/PID2023-149211OB-C31
000162409 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000162409 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000162409 700__ $$aFernández, Pablo S.
000162409 700__ $$aO'Byrne, Conor
000162409 700__ $$aGarre, Alberto
000162409 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000162409 773__ $$g221, Part. 1 (2025), 117165 [10 pp.]$$pFood res. int.$$tFood Research International$$x0963-9969
000162409 8564_ $$s2280342$$uhttps://zaguan.unizar.es/record/162409/files/texto_completo.pdf$$yVersión publicada
000162409 8564_ $$s2494522$$uhttps://zaguan.unizar.es/record/162409/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000162409 909CO $$ooai:zaguan.unizar.es:162409$$particulos$$pdriver
000162409 951__ $$a2025-10-17-14:14:13
000162409 980__ $$aARTICLE