000162717 001__ 162717
000162717 005__ 20251017144602.0
000162717 0247_ $$2doi$$a10.1016/j.crmicr.2025.100461
000162717 0248_ $$2sideral$$a145371
000162717 037__ $$aART-2025-145371
000162717 041__ $$aeng
000162717 100__ $$0(orcid)0000-0002-4915-3302$$aSalvador, Maika$$uUniversidad de Zaragoza
000162717 245__ $$aGenomic identification of germinant receptors and phenotypic response to germinants in Parageobacillus and Geobacillus spp. strains
000162717 260__ $$c2025
000162717 5060_ $$aAccess copy available to the general public$$fUnrestricted
000162717 5203_ $$aBacterial spore germination is triggered by specific nutrients detected by germinant receptors (GRs) located in the inner membrane. While GR diversity and function are well-characterized in some Bacillus and Clostridium spp., they remain poorly understood in (Para)Geobacillus spp., despite the relevance of their spores in food spoilage and biotechnology. This study analyzed 105 genomes from (Para)Geobacillus strains to identify GR-encoding operons and evaluated the germination of 22 representative strains. All strains carried between two and five GRs, mostly orthologous to GRs in B. subtilis, Priestia megaterium, B. cereus, and B. anthracis, with wide variation among species and in some cases, such as G. stearothermophilus, among strains. Among GRs, the most commonly conserved GR across all strains was an ortholog of GerK from B. subtilis 168 and Priestia megaterium QM B1551. All strains germinated in rich nutrient medium (TSBYE), but none responded to common amino acids or nucleosides, and only a few (G. stearothermophilus ATCC 12980T and ATCC 10149, G. thermodenitrificans DSM 465T, and several P. thermoglucosidasius strains) germinated in the presence of various sugars. Notably, differences in germination responses did not align with GR diversity. Additionally, all strains displayed poor outgrowth on rich nutrient agar, with colony formation efficiencies ranging from 3 % to 53 %. These findings suggest that germination and outgrowth mechanisms in (Para)Geobacillus differ significantly from those in well-studied spore-formers, highlighting the need for further research.
000162717 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FEDER/E35-23R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2023-148505OB-I00
000162717 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000162717 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000162717 700__ $$0(orcid)0000-0003-3608-4720$$aYruela, Inmaculada
000162717 700__ $$0(orcid)0000-0002-1902-0648$$aCondón, Santiago$$uUniversidad de Zaragoza
000162717 700__ $$0(orcid)0000-0002-5895-2157$$aGayán, Elisa$$uUniversidad de Zaragoza
000162717 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000162717 773__ $$g9 (2025), 100461 [17 pp.]$$tCurrent Research in Microbial Sciences$$x2666-5174
000162717 8564_ $$s11658553$$uhttps://zaguan.unizar.es/record/162717/files/texto_completo.pdf$$yVersión publicada
000162717 8564_ $$s2667559$$uhttps://zaguan.unizar.es/record/162717/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000162717 909CO $$ooai:zaguan.unizar.es:162717$$particulos$$pdriver
000162717 951__ $$a2025-10-17-14:14:25
000162717 980__ $$aARTICLE