000162823 001__ 162823
000162823 005__ 20251017144653.0
000162823 0247_ $$2doi$$a10.1016/j.jhazmat.2025.139384
000162823 0248_ $$2sideral$$a145238
000162823 037__ $$aART-2025-145238
000162823 041__ $$aeng
000162823 100__ $$aAsghar, Muhammad Umer
000162823 245__ $$aA metagenomics-based approach to understanding the transmission of healthcare-associated antimicrobial resistance in Pakistan
000162823 260__ $$c2025
000162823 5060_ $$aAccess copy available to the general public$$fUnrestricted
000162823 5203_ $$aHospital environments are critical yet underexamined reservoirs for hazardous antimicrobial resistance (AMR), particularly in lower-middle-income countries (LMICs) where resource constraints often hinder comprehensive surveillance. In this study, we employed 16S rRNA gene sequencing and shotgun metagenomics to characterize the microbiome, resistome, and potential transmission routes across five clinical environments within a hospital in Pakistan: the intensive care unit (ICU), surgical ward (SW), cardiac surgery ward (CSW), cardiac ward (CW), and operating theater (OT). Microbial community analysis revealed compositional similarities among the ICU, SW, and OT, with the ICU emerging as a primary source of microbial dissemination. Species-level profiling identified hospital-associated pathogens such as Acinetobacter baumannii, Klebsiella pneumoniae, and Enterobacter cloacae, and metagenome-assembled genome (MAG) analysis enabled the linkage of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) to specific bacterial hosts. ARGs and MGEs displayed setting-specific patterns, with the SW harboring the highest abundance of ARGs, particularly those conferring resistance to β-lactams and biocides. Insertion sequences were the most prevalent MGEs and were commonly linked to ARGs, indicating potential horizontal gene transfer. Co-occurrence network analysis identified Staphylococcus, Enterococcus, and Escherichia as central hub taxa within the microbial communities of the ICU, SW, and OT, indicating their critical roles in potential ARG transmission. These findings provide critical insights into the environmental transmission dynamics of AMR in LMIC healthcare settings and underscore the urgent need for metagenomics-informed infection control strategies.
000162823 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000162823 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000162823 700__ $$aZhai, Yuting
000162823 700__ $$aLiu, Ting
000162823 700__ $$aFan, Peixin
000162823 700__ $$aAin, Noor Ul
000162823 700__ $$aZaidi, Arsalan Haseeb
000162823 700__ $$aTariq, Muhammad
000162823 700__ $$0(orcid)0000-0001-5442-7702$$aMainar-Jaime, Raul C.$$uUniversidad de Zaragoza
000162823 700__ $$aJeong, Kwangcheol C.
000162823 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000162823 773__ $$g496 (2025), 139384 [11 pp.]$$pJ. hazard. mater.$$tJournal of Hazardous Materials$$x0304-3894
000162823 8564_ $$s5013233$$uhttps://zaguan.unizar.es/record/162823/files/texto_completo.pdf$$yVersión publicada
000162823 8564_ $$s1951039$$uhttps://zaguan.unizar.es/record/162823/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000162823 909CO $$ooai:zaguan.unizar.es:162823$$particulos$$pdriver
000162823 951__ $$a2025-10-17-14:37:09
000162823 980__ $$aARTICLE