000162829 001__ 162829
000162829 005__ 20251017144653.0
000162829 0247_ $$2doi$$a10.3389/fbioe.2025.1625999
000162829 0248_ $$2sideral$$a145374
000162829 037__ $$aART-2025-145374
000162829 041__ $$aeng
000162829 100__ $$aMorini, Sara
000162829 245__ $$aIsolation, characterization, and vascular potential of porcine cells in a three-dimensional decellularized liver matrix model
000162829 260__ $$c2025
000162829 5060_ $$aAccess copy available to the general public$$fUnrestricted
000162829 5203_ $$aIntroduction: The bioengineering of solid tissues and organs to mitigate the organ donor shortage has become a critical area of research in tissue engineering and regenerative medicine, where establishing a functional vascular network is crucial, particularly for complex organs such as the lung, kidney, and liver. This requires the isolation and characterization of various vascular cell types. In this quest, pigs have emerged as the preferred experimental animal model in this field, highlighting the importance of procuring and characterizing porcine vascular cells to create organs with functional vasculature for transplant. However, species-unique differences present challenges. Although some of the processes for isolating, expanding, and characterizing porcine vascular cells have been published, these are less established than those for human cells, requiring in our view and experience, additional research. Furthermore, no reliable and comprehensive models currently exist for testing vascular cell interactions in co-culture in vitro.MethodsIn this study, we developed effective methods to isolate and further characterize distinct porcine vascular cell types from various sources. We also introduced a straightforward and practical three-dimensional model for testing vascular cell co-culture, organization and function in vitro.Results and discussionThis proof-of-concept study demonstrates the potential of our co-culture strategy, employing a decellularized liver extracellular matrix disc scaffold microenvironment to assess cell interactions and vascular potential on a small scale in vitro.
Methods: In this study, we developed effective methods to isolate and further characterize distinct porcine vascular cell types from various sources. We also introduced a straightforward and practical three-dimensional model for testing vascular cell co-culture, organization and function in vitro.
Results and discussion: This proof-of-concept study demonstrates the potential of our co-culture strategy, employing a decellularized liver extracellular matrix disc scaffold microenvironment to assess cell interactions and vascular potential on a small scale in vitro.
000162829 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/PI15-00563$$9info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI18-00529$$9info:eu-repo/grantAgreement/ES/ISCIII/PI21-00667
000162829 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000162829 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000162829 700__ $$aMelitón Barbancho, Sandra
000162829 700__ $$aBlanes Rodríguez, Álvaro
000162829 700__ $$aPla-Palacín, Iris
000162829 700__ $$aSainz-Arnal, Pilar
000162829 700__ $$aSánchez-Romero, Natalia
000162829 700__ $$0(orcid)0000-0001-6964-7011$$aFalceto, Maria Victoria$$uUniversidad de Zaragoza
000162829 700__ $$0(orcid)0000-0001-9907-2941$$aMitjana, Olga$$uUniversidad de Zaragoza
000162829 700__ $$0(orcid)0000-0001-7188-0461$$aRomero, Antonio$$uUniversidad de Zaragoza
000162829 700__ $$aDel Rio-Nechaevsky, Marcela
000162829 700__ $$0(orcid)0000-0002-4575-9067$$aBengochea Martinez, Maria Lourdes$$uUniversidad de Zaragoza
000162829 700__ $$aOlmedo Arbizu, Emma
000162829 700__ $$0(orcid)0000-0003-4672-8083$$aLorente, Sara$$uUniversidad de Zaragoza
000162829 700__ $$0(orcid)0000-0001-5932-2889$$aLanas, Angel$$uUniversidad de Zaragoza
000162829 700__ $$aFernandes-Platzgummer, Ana
000162829 700__ $$aBaptista, Pedro M.
000162829 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina
000162829 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000162829 7102_ $$11013$$2020$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Anatomía Patológica
000162829 773__ $$g13 (2025), [17 pp.]$$pFront. Bioeng. Biotechnol.$$tFrontiers in Bioengineering and Biotechnology$$x2296-4185
000162829 8564_ $$s3879325$$uhttps://zaguan.unizar.es/record/162829/files/texto_completo.pdf$$yVersión publicada
000162829 8564_ $$s2332838$$uhttps://zaguan.unizar.es/record/162829/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000162829 909CO $$ooai:zaguan.unizar.es:162829$$particulos$$pdriver
000162829 951__ $$a2025-10-17-14:37:11
000162829 980__ $$aARTICLE