162829 20260205143932.0 doi 10.3389/fbioe.2025.1625999 sideral 145374 ART-2025-145374 eng Morini, Sara Isolation, characterization, and vascular potential of porcine cells in a three-dimensional decellularized liver matrix model 2025 Access copy available to the general public Unrestricted Introduction: The bioengineering of solid tissues and organs to mitigate the organ donor shortage has become a critical area of research in tissue engineering and regenerative medicine, where establishing a functional vascular network is crucial, particularly for complex organs such as the lung, kidney, and liver. This requires the isolation and characterization of various vascular cell types. In this quest, pigs have emerged as the preferred experimental animal model in this field, highlighting the importance of procuring and characterizing porcine vascular cells to create organs with functional vasculature for transplant. However, species-unique differences present challenges. Although some of the processes for isolating, expanding, and characterizing porcine vascular cells have been published, these are less established than those for human cells, requiring in our view and experience, additional research. Furthermore, no reliable and comprehensive models currently exist for testing vascular cell interactions in co-culture in vitro.MethodsIn this study, we developed effective methods to isolate and further characterize distinct porcine vascular cell types from various sources. We also introduced a straightforward and practical three-dimensional model for testing vascular cell co-culture, organization and function in vitro.Results and discussionThis proof-of-concept study demonstrates the potential of our co-culture strategy, employing a decellularized liver extracellular matrix disc scaffold microenvironment to assess cell interactions and vascular potential on a small scale in vitro. Methods: In this study, we developed effective methods to isolate and further characterize distinct porcine vascular cell types from various sources. We also introduced a straightforward and practical three-dimensional model for testing vascular cell co-culture, organization and function in vitro. Results and discussion: This proof-of-concept study demonstrates the potential of our co-culture strategy, employing a decellularized liver extracellular matrix disc scaffold microenvironment to assess cell interactions and vascular potential on a small scale in vitro. info:eu-repo/grantAgreement/ES/ISCIII/PI15-00563 info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI18-00529 info:eu-repo/grantAgreement/ES/ISCIII/PI21-00667 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Melitón Barbancho, Sandra Blanes Rodríguez, Álvaro Pla-Palacín, Iris Sainz-Arnal, Pilar Sánchez-Romero, Natalia Falceto, Maria Victoria Universidad de Zaragoza (orcid)0000-0001-6964-7011 Mitjana, Olga Universidad de Zaragoza (orcid)0000-0001-9907-2941 Romero, Antonio Universidad de Zaragoza (orcid)0000-0001-7188-0461 Del Rio-Nechaevsky, Marcela Bengochea Martinez, Maria Lourdes Universidad de Zaragoza (orcid)0000-0002-4575-9067 Olmedo Arbizu, Emma Lorente, Sara Universidad de Zaragoza (orcid)0000-0003-4672-8083 Lanas, Angel Universidad de Zaragoza (orcid)0000-0001-5932-2889 Fernandes-Platzgummer, Ana Baptista, Pedro M. 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 1013 020 Universidad de Zaragoza Dpto. Cirugía Área Anatomía Patológica 13 (2025), [17 pp.] Front. Bioeng. Biotechnol. Frontiers in Bioengineering and Biotechnology 2296-4185 3879325 http://zaguan.unizar.es/record/162829/files/texto_completo.pdf Versión publicada 2332838 http://zaguan.unizar.es/record/162829/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:162829 articulos driver 2026-02-05-14:38:53 ARTICLE