000162946 001__ 162946
000162946 005__ 20251017144637.0
000162946 0247_ $$2doi$$a10.1016/j.rvsc.2025.105888
000162946 0248_ $$2sideral$$a145399
000162946 037__ $$aART-2025-145399
000162946 041__ $$aeng
000162946 100__ $$0(orcid)0000-0001-5084-6555$$aGarcía-Belenguer, Sylvia$$uUniversidad de Zaragoza
000162946 245__ $$aEffect of Lactobacillus supplementation on seizure control, gut microbiota, and blood neurotransmitters in dogs with idiopathic epilepsy
000162946 260__ $$c2025
000162946 5060_ $$aAccess copy available to the general public$$fUnrestricted
000162946 5203_ $$aIdiopathic epilepsy is one of the most common neurological diseases in dogs; however, epileptogenesis and the mechanisms underlying drug resistance remain poorly understood. This study aimed to evaluate the effects of three months of supplementation with canine-derived Lactobacillaceae strains on seizure frequency in dogs with drug-sensitive epilepsy (DSE) and drug-refractory epilepsy (DRE). Additionally, gut microbiota profiles and plasma levels of GABA, L-glutamate, and serotonin were analysed and compared to those of healthy control dogs.
The probiotic supplementation was associated with a reduction in both the number of seizures and seizure days in epileptic dogs. An increase in the relative abundance of gut Lactobacillus and plasma GABA levels did not accompany clinical improvement. However, it was associated with a significant decrease in the GABA/L-Glutamate ratio in dogs with DSE but not in those with DRE. At baseline, heterogeneity of the fecal microbial community (β-diversity) was higher in epileptic compared to controls. Following probiotic supplementation, the differences in β-diversity between control and DSE dogs were no longer observed. The probiotic had no notable effect on the control group but was associated with a significant reduction in β-diversity in dogs with DRE.
While the relative abundance of Lactobacillus remained unchanged after probiotic administration, some changes in gut microbiota were observed in epileptic dogs. Numerous associations were identified between seizure frequency and specific bacterial taxa with potential protective effects (e.g.,Prevotella_9, Ligilactobacillus) or risk effects (e.g.,Parasutterella, Helicobacter). These findings suggest that long-term use of Lactobacillus may serve as a promising adjuvant strategy for seizure control.
000162946 536__ $$9info:eu-repo/grantAgreement/ES/DGA/LMP134-21
000162946 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000162946 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000162946 700__ $$aRosado, Belén$$uUniversidad de Zaragoza
000162946 700__ $$0(orcid)0000-0002-6806-9990$$aHernaiz, Adelaida$$uUniversidad de Zaragoza
000162946 700__ $$aMoral, Jon
000162946 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, Inmaculada$$uUniversidad de Zaragoza
000162946 700__ $$0(orcid)0000-0002-1581-3085$$aPalacio, Jorge$$uUniversidad de Zaragoza
000162946 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000162946 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000162946 773__ $$g196 (2025), 105888 [10 pp.]$$pRes. Vet. Sci.$$tRESEARCH IN VETERINARY SCIENCE$$x0034-5288
000162946 8564_ $$s1324409$$uhttps://zaguan.unizar.es/record/162946/files/texto_completo.pdf$$yVersión publicada
000162946 8564_ $$s2627328$$uhttps://zaguan.unizar.es/record/162946/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000162946 909CO $$ooai:zaguan.unizar.es:162946$$particulos$$pdriver
000162946 951__ $$a2025-10-17-14:30:04
000162946 980__ $$aARTICLE