doi:10.1016/j.vas.2025.100505engFalceto, María VictoriaReyero, DavidTejedor, María TeresaRenaud, PaulMateu, AnaLafuente, AntonioBonastre, CristinaLaborda, AliciaGarrido, Ana MariaMitjana, OlgaRole of triptorelin and single fixed-time artificial insemination on productive and reproductive performance of hyperprolific sowsART-2025-145435Single fixed-time artificial insemination (SFTAI) provides a streamlined approach to improving reproductive management. We evaluated the impact of incorporating triptorelin acetate (GnRH agonist) into a SFTAI protocol on a commercial farm. Hyperprolific sows not in estrus by day 4 post-weaning were randomly assigned to two groups. Group-1 (G1; n = 243) received no triptorelin treatment and daily post-cervical artificial inseminations (PCAI) based on estrus detection (2.59±0.034 inseminations/sow). Group-2 (G2; n = 249) received a single intravaginal dose of triptorelin (0.2 mg) at 96 ± 2 h post-weaning, then one PCAI 22 ± 2 h later. No significant differences (P > 0.05) between groups in conception rate (G1: 98.8 %; G2: 97.6 %), farrowing rate (G1:95.1 %; G2: 94.4 %) or litter characteristics (total born, live-, stillborn and mummified piglets). Both groups showed similar delivery batch durations (P = 0.414). Distribution of deliveries during the delivery period was similar (P = 0.455). Induction of labor was need more frequently in G1 (P < 0.001). G2 had a higher proportion of sows with shorter gestations (112–115 days: 166/235, 70.6 %) compared to G1 (112–115 days: 82/231; 35.5 %, P < 0.001). A significantly higher proportion of G2 piglets received longer lactation (27 - 33 days) compared to G1 (2210/3023=73.1 % vs 2774/4395=63.1 %; P = 0.032). G1 had a higher proportion of low-weight piglets (≤4.0 kg; 716/43950= 16.3 % vs.435/3023= 14.4 %; while G2 had more heavy piglets (≥6.5 kg; 1228/3023=40.5 % vs.1675/4395= 38.1 %) (P = 0.027). Observed differences in lactation length could be responsible for these weaning weight differences, rather than triptorelin treatment itself. This protocol did not worsen productive and reproductive performances.2025http://zaguan.unizar.es/record/16295010.1016/j.vas.2025.100505http://zaguan.unizar.es/record/162950oai:zaguan.unizar.es:162950Veterinary and Animal Science 30 (2025), 100505 [10 pp.]by-nc-ndhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.esinfo:eu-repo/semantics/openAccess