000163006 001__ 163006
000163006 005__ 20251009133841.0
000163006 0247_ $$2doi$$a10.1016/j.cimid.2025.102404
000163006 0248_ $$2sideral$$a145542
000163006 037__ $$aART-2025-145542
000163006 041__ $$aeng
000163006 100__ $$0(orcid)0000-0001-5608-781X$$aMillán, Javier
000163006 245__ $$aMolecular screening for blood pathogens in synanthropic Pipistrellus bats in Spain reveals novel and human-related hemoplasmas
000163006 260__ $$c2025
000163006 5060_ $$aAccess copy available to the general public$$fUnrestricted
000163006 5203_ $$aUrbanization profoundly alters natural habitats, creating environments where adaptable species such as bats thrive. In developed countries, cities may act as hotspots for pathogen transmission from bats to humans, yet urban bat pathogens remain understudied in Europe. This study examined vector-borne and zoonotic bacteria and protozoa in soprano (Pipistrellus pygmaeus, PPY) and Kuhl’s pipistrelles (Pipistrellus kuhlii, PKU) inhabiting Zaragoza, Spain. A total of 213 bats (143 urban, 70 rural; including 7 recaptures) were live-trapped between 2022 and 2024, and blood samples were collected. Initial screening of 77 individuals for Anaplasmataceae, Bartonella, Borrelia, hemotropic Mycoplasma (hemoplasmas), and Leishmania revealed hemoplasmas as the only haemopathogens present. Sequencing of a 330-bp 16S rRNA fragment confirmed infection, which was subsequently assessed in the full sample. Overall, ten bats (4.69 %) tested positive: eight PPY (two rural, six urban) and two PKU (one rural, one urban). Two hemoplasma genotypes were identified. The first, detected in both species across habitats, showed similarity to sequences from bats in Germany and Chile. The second, found in PPY from both environments, clustered closely with the human hemolytic pathogen Candidatus Mycoplasma haematohominis. Extended 16S rRNA (∼ 1400 bp) and 23S rRNA (∼ 1100 bp) sequences were obtained only for the first genotype, which phylogenetic analyses indicated represents a novel species. Since both samples had 99.8–100 % sequence identity across markers, we propose naming it Candidatus Mycoplasma haematopipistrellus sp. nov. Despite the low pathogen diversity observed, results highlight bats as potential ecological bridges for hemoplasma transmission between rural and urban environments.
000163006 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A16-23R$$9info:eu-repo/grantAgreement/ES/UZ/2021-BIO-03
000163006 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttps://creativecommons.org/licenses/by-nc/4.0/deed.es
000163006 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000163006 700__ $$aSantibáñez, Paula
000163006 700__ $$0(orcid)0000-0003-0210-2919$$aMonteagudo, Luis Vicente$$uUniversidad de Zaragoza
000163006 700__ $$aSoares, Sofia M.
000163006 700__ $$aIsrael, Alberto
000163006 700__ $$0(orcid)0000-0001-8435-6386$$aRodríguez-Pastor, Ruth
000163006 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000163006 773__ $$g124 (2025), 102404 [8 pp.]$$pComp. immunol. microbiol. infect. dis.$$tCOMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES$$x0147-9571
000163006 8564_ $$s8731451$$uhttps://zaguan.unizar.es/record/163006/files/texto_completo.pdf$$yVersión publicada
000163006 8564_ $$s2609943$$uhttps://zaguan.unizar.es/record/163006/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000163006 909CO $$ooai:zaguan.unizar.es:163006$$particulos$$pdriver
000163006 951__ $$a2025-10-09-13:25:56
000163006 980__ $$aARTICLE