000163069 001__ 163069
000163069 005__ 20251009133841.0
000163069 0247_ $$2doi$$a10.3390/ani15172632
000163069 0248_ $$2sideral$$a145530
000163069 037__ $$aART-2025-145530
000163069 041__ $$aeng
000163069 100__ $$aReséndiz-Pozos, Raúl A.
000163069 245__ $$aHistopathological and Immunohistochemical Study of Neoplastic Cell Heterogeneity in Early and Advanced Ovine Pulmonary Adenocarcinoma
000163069 260__ $$c2025
000163069 5060_ $$aAccess copy available to the general public$$fUnrestricted
000163069 5203_ $$aOvine pulmonary adenocarcinoma (OPA) is a naturally occurring lung neoplasia in sheep caused by jaagsiekte sheep retrovirus (JSRV). JSRV infects alveolar type II pneumocytes (ATII) and club cells (CC), and the expression of viral oncoproteins induces a lung adenocarcinoma. The gross pathology of OPA exhibits differences in the anatomical patterns known as classical and atypical forms. Thirty natural OPA tumors, divided equally into early OPA tumors (Group A, GA), atypical tumors (Group B, GB), and classical tumors (Group C, GC), were obtained from adult sheep (2–9 years old). Tumor heterogeneity was studied comparing the histopathology (growth patterns, local invasion, mitotic figures, myxoid nodules), together with immunohistochemistry (IHC) using markers of JSRV-ENV, epithelial cells (ATII cells, CC, ki67), progenitor-stem epithelial cells (K5, p63, CD44), and the anterior grade protein 2 (AGR2). Papillary pattern was predominant in all groups. Lepidic pattern was also relevant in GA, and acinar pattern was relevant in GB. Low proliferation indexes and local invasion were observed in all groups. Myxoid nodules were few. IHC showed that all samples were positive for JSRV-ENV. Cell markers demonstrated that GA was different when compared to GB and GC, showing significantly the highest levels of CC, K5, and p63 positive tumor cells. There were no significant differences between GB and GC. The heterogeneity analysis of OPA tumors revealed that in early tumors, repair is important but is not reflected in classical or atypical different anatomical OPA forms.
000163069 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A15-23R
000163069 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000163069 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000163069 700__ $$0(orcid)0000-0002-4655-9171$$aGonzález-Saínz, Jose María$$uUniversidad de Zaragoza
000163069 700__ $$0(orcid)0000-0002-0313-5926$$aOrtín, Aurora$$uUniversidad de Zaragoza
000163069 700__ $$aAsin, Javier
000163069 700__ $$0(orcid)0000-0002-0606-0763$$aCliment, María$$uUniversidad de Zaragoza
000163069 700__ $$0(orcid)0000-0003-3296-9644$$aBorderías, Luis$$uUniversidad de Zaragoza
000163069 700__ $$0(orcid)0000-0003-3792-287X$$aHeras, Marcelo de las$$uUniversidad de Zaragoza
000163069 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000163069 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000163069 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina
000163069 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000163069 773__ $$g15, 17 (2025), 2632 [17 pp.]$$pAnimals (Basel)$$tAnimals$$x2076-2615
000163069 8564_ $$s2164276$$uhttps://zaguan.unizar.es/record/163069/files/texto_completo.pdf$$yVersión publicada
000163069 8564_ $$s2738958$$uhttps://zaguan.unizar.es/record/163069/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000163069 909CO $$ooai:zaguan.unizar.es:163069$$particulos$$pdriver
000163069 951__ $$a2025-10-09-13:25:56
000163069 980__ $$aARTICLE