000163183 001__ 163183 000163183 005__ 20251017161900.0 000163183 0247_ $$2doi$$a10.1080/01652176.2025.2556492 000163183 0248_ $$2sideral$$a145664 000163183 037__ $$aART-2025-145664 000163183 041__ $$aeng 000163183 100__ $$aGómez, Álex 000163183 245__ $$aImmunogenicity, security and protection against small ruminant lentivirus (SRLV) challenge in sheep, induced by intranasal immunization with a recombinant Sendai virus vector expressing SRLV gag-P25 000163183 260__ $$c2025 000163183 5060_ $$aAccess copy available to the general public$$fUnrestricted 000163183 5203_ $$aSmall ruminant lentiviruses (SRLV) are responsible for significant economic losses in sheep and goat farming; however, effective vaccination strategies remain unavailable. This study evaluated the immunogenicity, safety, and protective efficacy of a recombinant Sendai virus vector (SeV) expressing SRLV gag-P25 (rSeV-GFP-P25) in lambs. Twenty-one SRLV-negative lambs were divided into three groups and inoculated intranasally thrice with culture medium (group 1); SeV-GFP (group 2) or rSeV-GFP-P25 (group 3). Lambs were challenged with homologous SRLV at 16 weeks post-first immunization. Clinical and hematological parameters, antibody responses, SRLV viral loads in peripheral blood mononuclear cells (PBMCs) and target tissues, histopathological and histomorphometric analyses, assisted with artificial intelligence, of interstitial pneumonia were assessed. No clinicopathological alterations were observed, except for a transient temperature increase in group 3 post-first immunization. Group 2 showed mild SeV-neutralizing antibodies, while rSeV-GFP-P25 (group 3) induced negligible SRLV-specific antibody responses. Group 3 exhibited higher SRLV DNA copies in PBMCs but lower in most SRLV target tissues compared to control groups, with no SRLV DNA detected in spleen and bone marrow. Histomorphometry revealed reduced alveolar septal thickening in group 3, indicating partial protection against early SRLV-associated interstitial pneumonia. These results warrant further investigation into cellular immunity and long-term protection. 000163183 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-096172-B-C33 000163183 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es 000163183 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000163183 700__ $$aGlaria, Idoia 000163183 700__ $$aMoncayola, Irati 000163183 700__ $$aEcheverría, Irache 000163183 700__ $$aRodríguez-Largo, Ana 000163183 700__ $$0(orcid)0000-0002-1204-4356$$ade Blas, Ignacio$$uUniversidad de Zaragoza 000163183 700__ $$0(orcid)0009-0003-6592-6306$$aPérez, Estela$$uUniversidad de Zaragoza 000163183 700__ $$0(orcid)0000-0002-8133-2124$$aPérez, Marta$$uUniversidad de Zaragoza 000163183 700__ $$0(orcid)0000-0001-6209-4282$$aVillanueva-Saz, Sergio$$uUniversidad de Zaragoza 000163183 700__ $$aLee, Benhur 000163183 700__ $$aDiego, Alicia de 000163183 700__ $$aMiguel, Ricardo de 000163183 700__ $$0(orcid)0000-0002-2053-9842$$aLuján, Lluís$$uUniversidad de Zaragoza 000163183 700__ $$aReina, Ramsés 000163183 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica 000163183 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000163183 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com 000163183 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal 000163183 773__ $$g45, 1 (2025), 16$$pVet. q.$$tVETERINARY QUARTERLY$$x0165-2176 000163183 8564_ $$s4803594$$uhttps://zaguan.unizar.es/record/163183/files/texto_completo.pdf$$yVersión publicada 000163183 8564_ $$s1485080$$uhttps://zaguan.unizar.es/record/163183/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000163183 909CO $$ooai:zaguan.unizar.es:163183$$particulos$$pdriver 000163183 951__ $$a2025-10-17-14:07:38 000163183 980__ $$aARTICLE