000163249 001__ 163249 000163249 005__ 20251024172258.0 000163249 0247_ $$2doi$$a10.1016/j.rvsc.2025.105916 000163249 0248_ $$2sideral$$a145753 000163249 037__ $$aART-2025-145753 000163249 041__ $$aeng 000163249 100__ $$0(orcid)0000-0002-6806-9990$$aHernaiz, Adelaida$$uUniversidad de Zaragoza 000163249 245__ $$aGut microbiota alterations linked to classical scrapie in sheep 000163249 260__ $$c2025 000163249 5060_ $$aAccess copy available to the general public$$fUnrestricted 000163249 5203_ $$aThere is growing evidence of the involvement of gut microbiota in neurodegenerative diseases. However, the relationship between gut microbiota and prion diseases is not yet well understood. Prion diseases are neurodegenerative disorders affecting humans and animals and caused by an infectious misfolded protein known as prion. Among prion diseases, scrapie is the one affecting sheep and goats, and its classical form is typically acquired. In the present work, a 16S rRNA-based microbiome profiling was performed in faecal samples from 10 sheep infected with classical scrapie and 10 control sheep. The phyla Firmicutes and Bacteroidetes appeared as the most abundant phyla in scrapie and control sheep. Moreover, both groups of samples showed similar richness and alpha diversity indices. The gut microbiota profile in terms of beta diversity was significantly different in scrapie-infected sheep compared to the control group. Additionally, similarly to other neurodegenerative diseases, scrapie animals displayed a significant increased abundance of Cyanobacteria and Lentisphaerae phyla. Our results point to an altered gut microbiota in sheep infected with classical scrapie, with a specific increase of two phyla that could be involved in scrapie disease pathogenesis, enabling conceptual advances in the understanding of prion diseases. 000163249 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A19-20R$$9info:eu-repo/grantAgreement/ES/DGA/A20-23R$$9info:eu-repo/grantAgreement/ES/NextGenerationEU/INVESTIGO-100-76 000163249 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es 000163249 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000163249 700__ $$0(orcid)0000-0002-5306-9365$$aGrasa, Laura$$uUniversidad de Zaragoza 000163249 700__ $$aDiego Abejón, Paula de 000163249 700__ $$aLeón Huertas, Celia 000163249 700__ $$0(orcid)0000-0002-1590-3347$$aMarín, Belén$$uUniversidad de Zaragoza 000163249 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan José$$uUniversidad de Zaragoza 000163249 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, Rosa$$uUniversidad de Zaragoza 000163249 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, Pilar$$uUniversidad de Zaragoza 000163249 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, Inmaculada$$uUniversidad de Zaragoza 000163249 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética 000163249 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología 000163249 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000163249 773__ $$g196 (2025), 105916 [9 pp.]$$pRes. Vet. Sci.$$tRESEARCH IN VETERINARY SCIENCE$$x0034-5288 000163249 8564_ $$s2479258$$uhttps://zaguan.unizar.es/record/163249/files/texto_completo.pdf$$yVersión publicada 000163249 8564_ $$s2479686$$uhttps://zaguan.unizar.es/record/163249/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000163249 909CO $$ooai:zaguan.unizar.es:163249$$particulos$$pdriver 000163249 951__ $$a2025-10-24-16:55:44 000163249 980__ $$aARTICLE