163249 20251024172258.0 doi 10.1016/j.rvsc.2025.105916 sideral 145753 ART-2025-145753 eng Hernaiz, Adelaida Universidad de Zaragoza (orcid)0000-0002-6806-9990 Gut microbiota alterations linked to classical scrapie in sheep 2025 There is growing evidence of the involvement of gut microbiota in neurodegenerative diseases. However, the relationship between gut microbiota and prion diseases is not yet well understood. Prion diseases are neurodegenerative disorders affecting humans and animals and caused by an infectious misfolded protein known as prion. Among prion diseases, scrapie is the one affecting sheep and goats, and its classical form is typically acquired. In the present work, a 16S rRNA-based microbiome profiling was performed in faecal samples from 10 sheep infected with classical scrapie and 10 control sheep. The phyla Firmicutes and Bacteroidetes appeared as the most abundant phyla in scrapie and control sheep. Moreover, both groups of samples showed similar richness and alpha diversity indices. The gut microbiota profile in terms of beta diversity was significantly different in scrapie-infected sheep compared to the control group. Additionally, similarly to other neurodegenerative diseases, scrapie animals displayed a significant increased abundance of Cyanobacteria and Lentisphaerae phyla. Our results point to an altered gut microbiota in sheep infected with classical scrapie, with a specific increase of two phyla that could be involved in scrapie disease pathogenesis, enabling conceptual advances in the understanding of prion diseases. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/DGA/A19-20R info:eu-repo/grantAgreement/ES/DGA/A20-23R info:eu-repo/grantAgreement/ES/NextGenerationEU/INVESTIGO-100-76 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Grasa, Laura Universidad de Zaragoza (orcid)0000-0002-5306-9365 Diego Abejón, Paula de León Huertas, Celia Marín, Belén Universidad de Zaragoza (orcid)0000-0002-1590-3347 Badiola, Juan José Universidad de Zaragoza (orcid)0000-0002-7173-7216 Bolea, Rosa Universidad de Zaragoza (orcid)0000-0002-2746-3932 Zaragoza, Pilar Universidad de Zaragoza (orcid)0000-0001-5740-0185 Martín-Burriel, Inmaculada Universidad de Zaragoza (orcid)0000-0001-6016-4726 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 196 (2025), 105916 [9 pp.] Res. Vet. Sci. RESEARCH IN VETERINARY SCIENCE 0034-5288 2479258 http://zaguan.unizar.es/record/163249/files/texto_completo.pdf Versión publicada 2479686 http://zaguan.unizar.es/record/163249/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:163249 articulos driver 2025-10-24-16:55:44 ARTICLE