000163298 001__ 163298
000163298 005__ 20251024172259.0
000163298 0247_ $$2doi$$a10.1039/d5na00404g
000163298 0248_ $$2sideral$$a145722
000163298 037__ $$aART-2025-145722
000163298 041__ $$aeng
000163298 100__ $$0(orcid)0000-0002-9181-2105$$aGimenez-Ingalaturre, Ana C.
000163298 245__ $$aSynergistic activity of silver nanoparticles and antibiotics: apramycin against <i>Escherichia coli</i>
000163298 260__ $$c2025
000163298 5060_ $$aAccess copy available to the general public$$fUnrestricted
000163298 5203_ $$aThe combination of silver nanoparticles and conventional antibiotics could be a promising alternative strategy to fight against antimicrobial resistance. The occurrence of synergistic bactericidal activity between these combinations is supported by a large number of studies, but there is still a great lack of information about the action mechanisms. In this study, a direct analytical method for the detection and quantification of silver content in cells based on single cell inductively coupled plasma mass spectrometry (SC-ICP-MS), in combination with microscopy and microbiological techniques, has been used. Quantification of silver accumulated in Escherichia coli bacteria exposed to combinations of silver(I) or silver nanoparticles and apramycin can help elucidate the synergistic mechanisms of silver–antibiotic combinations. The combination of silver with apramycin resulted in the occurrence of synergistic effects, allowing the reduction of silver(I), silver nanoparticle and apramycin concentrations (from 4- to 16-fold) while preserving the individual bactericidal effects of each antimicrobial. Severe damage in bacteria walls, including double membrane rupture and cytoplasm leakage, was observed when the combination of apramycin and silver was used. In general, the presence of apramycin has promoted silver uptake by bacteria.
000163298 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B43-23R$$9info:eu-repo/grantAgreement/ES/DGA/E29-23R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-123203OB-I00$$9info:eu-repo/grantAgreement/ES/NextGenerationEU/MZ-240621
000163298 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttps://creativecommons.org/licenses/by-nc/4.0/deed.es
000163298 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000163298 700__ $$0(orcid)0000-0002-7931-3901$$aAbad-Álvaro, Isabel$$uUniversidad de Zaragoza
000163298 700__ $$0(orcid)0000-0002-9981-9045$$aChueca, Patricia$$uUniversidad de Zaragoza
000163298 700__ $$0(orcid)0000-0003-0765-7227$$aGoñi, Pilar$$uUniversidad de Zaragoza
000163298 700__ $$0(orcid)0000-0002-4169-0357$$aLaborda, Francisco$$uUniversidad de Zaragoza
000163298 7102_ $$11011$$2660$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Parasitología
000163298 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000163298 7102_ $$11001$$2X$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Técnica. Lab. y Talleres
000163298 773__ $$g7, 19 (2025), 6120-6131$$tNanoscale Advances$$x2516-0230
000163298 8564_ $$s1551870$$uhttps://zaguan.unizar.es/record/163298/files/texto_completo.pdf$$yVersión publicada
000163298 8564_ $$s2940079$$uhttps://zaguan.unizar.es/record/163298/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000163298 909CO $$ooai:zaguan.unizar.es:163298$$particulos$$pdriver
000163298 951__ $$a2025-10-24-16:56:42
000163298 980__ $$aARTICLE