000163322 001__ 163322 000163322 005__ 20251024172259.0 000163322 0247_ $$2doi$$a10.3390/insects16090989 000163322 0248_ $$2sideral$$a145746 000163322 037__ $$aART-2025-145746 000163322 041__ $$aeng 000163322 100__ $$aFondevila, Guillermo$$uUniversidad de Zaragoza 000163322 245__ $$aEffect of Supplementing Lysine and Isoleucine as Limiting Amino Acids on Growth Performance and Amino Acid Deposition of Tenebrio molitor Larvae Reared on a Cereal-Based Substrate 000163322 260__ $$c2025 000163322 5060_ $$aAccess copy available to the general public$$fUnrestricted 000163322 5203_ $$aThe effects of Lys and Ile supplementation in a barley-based substrate on growth performance and nutrient deposition in Tenebrio molitor larvae were evaluated. Six trays per treatment containing sixty larvae were assigned to five treatments in two 21-day periods: barley (B); an 85% barley and 15% soybean meal mixture (BS) and B supplemented with synthetic Lys (BL), Ile (BI) or a combination of both (BLI), as potential limiting amino acids (AA) for larval growth. Supplementing AAs was provided in agar cubes twice per week. Larval performance was measured, and larvae were analyzed for nutrient content and AA profile. Final larval weight increased (p = 0.042) in BS and BL compared to B, with intermediate values for BI and BLI. Larval contents (mg/larva) in total AAs and seven key AAs (sum of Lys, Met, Cys, Thr, Trp, Val and Ile) were higher in BS-fed larvae than in BL and BLI, with the lowest values for BI and B (p < 0.001). Barley supplementation with Lys improved larval AA content, although the levels remained lower than those observed with BS. Conversely, Ile supplementation had no apparent effect on overall AA deposition compared to Lys alone, suggesting the presence of additional limiting AAs for optimal larval growth. 000163322 536__ $$9info:eu-repo/grantAgreement/ES/UZ-OTRI/2018-0406 000163322 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es 000163322 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000163322 700__ $$aFatmi, Habib 000163322 700__ $$aFernando, Pilar 000163322 700__ $$aDapoza, Carlos 000163322 700__ $$0(orcid)0000-0002-0712-1185$$aFondevila, Manuel$$uUniversidad de Zaragoza 000163322 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal 000163322 773__ $$g16, 9 (2025), 989 [12 pp.]$$pInsects$$tInsects$$x2075-4450 000163322 8564_ $$s235324$$uhttps://zaguan.unizar.es/record/163322/files/texto_completo.pdf$$yVersión publicada 000163322 8564_ $$s2608766$$uhttps://zaguan.unizar.es/record/163322/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000163322 909CO $$ooai:zaguan.unizar.es:163322$$particulos$$pdriver 000163322 951__ $$a2025-10-24-16:57:06 000163322 980__ $$aARTICLE