000163754 001__ 163754
000163754 005__ 20251030150827.0
000163754 0247_ $$2doi$$a10.1016/j.jmbbm.2025.107234
000163754 0248_ $$2sideral$$a145822
000163754 037__ $$aART-2025-145822
000163754 041__ $$aeng
000163754 100__ $$aFantaci, B.$$uUniversidad de Zaragoza
000163754 245__ $$aComparative evaluation of the mechanical properties of amniotic membranes for corneal grafting: Effects of cryopreservation, lyophilization, and dehydration
000163754 260__ $$c2025
000163754 5060_ $$aAccess copy available to the general public$$fUnrestricted
000163754 5203_ $$aIn both human and veterinary ophthalmology, stromal to full-thickness corneal defects can be treated using several techniques that involve various biomaterials, which support healing while preserving vision. Among these, the amniotic membrane stands out due to its multifaceted biological properties, including anti-inflammatory, antimicrobial, and regenerative capabilities. Three main preservation methods (cryopreservation, dehydration, and lyophilization) have been developed to facilitate amniotic membrane clinical use. However, these methods may significantly alter its mechanical and structural integrity, which are critical for graft success. This study investigates the mechanical performance of bovine amniotic membranes for veterinary use, preserved using the above-mentioned techniques, by means of uniaxial tensile and inflation tests in a non-biological in vitro setting. Structural and chemical differences are also analyzed using scanning electron microscopy and energy-dispersive X-ray spectroscopy. Despite structural differences arose among the samples, these differences did not alter or worsen the structural resistance of the membranes. All samples withstood 5 inflation cycles of 200 mmHg without leaking.
000163754 536__ $$9info:eu-repo/grantAgreement/ES/DGA/T24-23R$$9info:eu-repo/grantAgreement/EC/H2020/956720/EU/Opto-Biomechanical Eye Research Network/OBERON$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 956720-OBERON$$9info:eu-repo/grantAgreement/ES/UZ/ICTS NANBIOSIS-U27 Unit-CIBER-BBN
000163754 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000163754 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000163754 700__ $$0(orcid)0000-0003-2361-1077$$aCalvo, B.$$uUniversidad de Zaragoza
000163754 700__ $$aMesa, A.M.
000163754 700__ $$aOrtillés, A.
000163754 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000163754 7102_ $$15008$$2250$$aUniversidad de Zaragoza$$bDpto. Ingeniería Electrón.Com.$$cÁrea Electrónica
000163754 773__ $$g173 (2025), 107234 [10 pp.]$$pJ. mech. behav. boomed. mater.$$tJournal of the Mechanical Behavior of Biomedical Materials$$x1751-6161
000163754 8564_ $$s4348611$$uhttps://zaguan.unizar.es/record/163754/files/texto_completo.pdf$$yVersión publicada
000163754 8564_ $$s2761615$$uhttps://zaguan.unizar.es/record/163754/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000163754 909CO $$ooai:zaguan.unizar.es:163754$$particulos$$pdriver
000163754 951__ $$a2025-10-30-14:40:18
000163754 980__ $$aARTICLE