Fabrication and characterization of an agarose and GelMA hybrid scaffold for adipose tissue regeneration
Teixeira, Ana Margarida ; Van Damme, Lana ; Van Vlierberghe, Sandra ; de Sousa Martins, Pedro Alexandre Lopes
Resumen: Purpose
This study aims to overcome the drawbacks of current clinical practices in breast reconstruction. Tissue engineering is currently the most promising field to create improved alternatives and new strategies. Biomaterials that mimic the physico-chemical and biological properties of the native extracellular matrix (ECM), e.g. hydrogels, have been characterized and seeded with cells, with the purpose of promoting the regeneration of tissues. In this study, a new hydrogel blend was produced and characterized for adipose tissue regeneration purposes.
Design/methodology/approach
Blends of agarose and gelatin-methacryloyl (GelMA) were produced in different weight percentage ratios (100:0, 75:25 and 50:50 of agarose:GelMA). Indirect 3D printed scaffolds and 2D discs were fabricated and characterized in terms of physico-chemical and biological properties in the presence of adipose tissue-derived stem cells.
Findings
Both scaffolds and discs presented a good gel fraction (>90% for most of the samples) and a swelling degree >17% for all samples. In terms of stiffness, the results varied from 2.86 kPa to 25.11 kPa depending on the testing conditions (i.e. testing temperature) and sample chemical composition. Rheology analysis showed that the storage modulus was higher than the loss modulus, indicating a more elastic behavior. Moreover, the scaffolds with GelMA could support cell adhesion and proliferation, with a viability higher than the control (i.e. tissue culture plastic, TCP) by day 14 (118% and 128% for the ratios 75:25 and 50:50, respectively), showing that 50:50 is the optimum ratio composition.
Originality/value
The blend of agarose GelMA presented in this study offers a new and promising approach toward adipose tissue regeneration.
Idioma: Inglés
DOI: 10.1108/RPJ-11-2024-0491
Año: 2025
Publicado en: RAPID PROTOTYPING JOURNAL 31, 9 (2025), 1933-1947
ISSN: 1355-2546
Tipo y forma: Artículo (PostPrint)
Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. No puede utilizar el material para una finalidad comercial.
Exportado de SIDERAL (2025-11-07-10:25:28)
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This study aims to overcome the drawbacks of current clinical practices in breast reconstruction. Tissue engineering is currently the most promising field to create improved alternatives and new strategies. Biomaterials that mimic the physico-chemical and biological properties of the native extracellular matrix (ECM), e.g. hydrogels, have been characterized and seeded with cells, with the purpose of promoting the regeneration of tissues. In this study, a new hydrogel blend was produced and characterized for adipose tissue regeneration purposes.
Design/methodology/approach
Blends of agarose and gelatin-methacryloyl (GelMA) were produced in different weight percentage ratios (100:0, 75:25 and 50:50 of agarose:GelMA). Indirect 3D printed scaffolds and 2D discs were fabricated and characterized in terms of physico-chemical and biological properties in the presence of adipose tissue-derived stem cells.
Findings
Both scaffolds and discs presented a good gel fraction (>90% for most of the samples) and a swelling degree >17% for all samples. In terms of stiffness, the results varied from 2.86 kPa to 25.11 kPa depending on the testing conditions (i.e. testing temperature) and sample chemical composition. Rheology analysis showed that the storage modulus was higher than the loss modulus, indicating a more elastic behavior. Moreover, the scaffolds with GelMA could support cell adhesion and proliferation, with a viability higher than the control (i.e. tissue culture plastic, TCP) by day 14 (118% and 128% for the ratios 75:25 and 50:50, respectively), showing that 50:50 is the optimum ratio composition.
Originality/value
The blend of agarose GelMA presented in this study offers a new and promising approach toward adipose tissue regeneration.
Idioma: Inglés
DOI: 10.1108/RPJ-11-2024-0491
Año: 2025
Publicado en: RAPID PROTOTYPING JOURNAL 31, 9 (2025), 1933-1947
ISSN: 1355-2546
Tipo y forma: Artículo (PostPrint)
Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. No puede utilizar el material para una finalidad comercial.Exportado de SIDERAL (2025-11-07-10:25:28)
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Registro creado el 2025-11-07, última modificación el 2025-11-07