000163846 001__ 163846
000163846 005__ 20251113150204.0
000163846 0247_ $$2doi$$a10.1016/j.actatropica.2025.107861
000163846 0248_ $$2sideral$$a146061
000163846 037__ $$aART-2025-146061
000163846 041__ $$aeng
000163846 100__ $$aValente Bertozzo, Thainá
000163846 245__ $$aFirst detection of Coxiella burnetii subtype 61 in bulk tank milk from dairy farms in Brazil
000163846 260__ $$c2025
000163846 5203_ $$aCoxiella burnetii (C. burnetii) is a Gram-negative bacterium that causes a cosmopolitan zoonosis called Q fever. C. burnetii infects a range of animals, but domestic ruminants (sheep, goats, and cattle) are the main reservoir for human infection. Coxiellosis is associated with reproductive disorders in animals, such as miscarriage, stillbirths, premature births, and infertility, posing a significant negative economic impact. We investigated the presence of C. burnetii in bulk tank milk samples from small family-operated farms in the Central West region of São Paulo, Brazil. Polymerase chain reaction (PCR), Nested PCR, and real-time PCR (qPCR- Sybr and Probe) followed by sequencing of products were performed. Additionally, we mapped the distribution of positive cases diagnosed in the different regions studied. C. burnetii was detected in 16/102 (15.68 %) by Nested-PCR, 22/102 (21.57 %) by qPCR/Sybr, and 32/102 (31.37 %) by qPCR/Probe. The agreement between methods was considered reasonable according to the Kappa analysis. C. burnetii was detected in seven of the eight cities investigated (87.50 %). All C. burnetii were ST61. Our findings indicate that C. burnetii is prevalent among family-operated dairy farms in this region of Brazil, underscoring the urgent need to develop comprehensive surveillance and control programs to mitigate the zoonotic risks associated with this bacterium.
000163846 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000163846 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000163846 700__ $$aManzini, Suzane
000163846 700__ $$aNeves Aires, Isabella
000163846 700__ $$aBezerra Bertolini, Amanda
000163846 700__ $$aMerino de Medeiros, Maria Izabel
000163846 700__ $$aBodelão Richini Pereira, Virgínia
000163846 700__ $$aBorin Nóbrega, Diego
000163846 700__ $$aRibeiro Mioni, Mateus de Souza
000163846 700__ $$aGregory, Lilian
000163846 700__ $$0(orcid)0000-0002-6282-5038$$aRamo Gil, María Ángeles$$uUniversidad de Zaragoza
000163846 700__ $$aPaes, Antonio Carlos
000163846 700__ $$aBaldini Lucheis, Simone
000163846 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000163846 773__ $$g271 (2025), 107861 [6 pp.]$$pActa Trop.$$tACTA TROPICA$$x0001-706X
000163846 8564_ $$s1284824$$uhttps://zaguan.unizar.es/record/163846/files/texto_completo.pdf$$yVersión publicada
000163846 8564_ $$s2395910$$uhttps://zaguan.unizar.es/record/163846/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000163846 909CO $$ooai:zaguan.unizar.es:163846$$particulos$$pdriver
000163846 951__ $$a2025-11-13-15:00:46
000163846 980__ $$aARTICLE